{"id":40957,"date":"2023-02-26T22:59:23","date_gmt":"2023-02-26T14:59:23","guid":{"rendered":"https:\/\/swarma.org\/?p=40957"},"modified":"2023-02-26T22:59:23","modified_gmt":"2023-02-26T14:59:23","slug":"%e5%89%8d%e6%b2%bf%e8%bf%9b%e5%b1%95%ef%bc%9aatp%e6%b0%b4%e5%b9%b3%e4%b8%8e%e6%b0%b4%e8%a7%a3%e8%87%aa%e7%94%b1%e8%83%bd%e6%80%8e%e6%a0%b7%e5%85%b1%e5%90%8c%e5%bd%b1%e5%93%8d%e7%bb%86%e8%83%9e","status":"publish","type":"post","link":"https:\/\/swarma.org\/?p=40957","title":{"rendered":"\u524d\u6cbf\u8fdb\u5c55\uff1aATP\u6c34\u5e73\u4e0e\u6c34\u89e3\u81ea\u7531\u80fd\u600e\u6837\u5171\u540c\u5f71\u54cd\u7ec6\u80de\u7684\u9499\u4fe1\u53f7\u8c03\u63a7\u7cfb\u7edf\u8d70\u5411\u201c\u5206\u53c9\u201d"},"content":{"rendered":"
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\u751f\u547d\u662f\u975e\u5e73\u8861\u6001\u7684\u5f00\u653e\u590d\u6742\u7cfb\u7edf\uff0c\u5176\u6709\u5e8f\u6027\u4f9d\u8d56\u4e8e\u5916\u90e8\u80fd\u91cf\u6444\u5165\u3002ATP\u6c34\u89e3\u53cd\u5e94\u662f\u751f\u547d\u6d3b\u52a8\u7684\u4e3b\u8981\u80fd\u91cf\u6765\u6e90\u3002\u4ece\u6c34\u89e3\u81ea\u7531\u80fd\u548c\u7ec6\u80de\u5185ATP\u6c34\u5e73\u89d2\u5ea6\u5c55\u5f00\u5b9a\u91cf\u7814\u7a76\uff0c\u5bf9\u751f\u547d\u590d\u6742\u7cfb\u7edf\u7814\u7a76\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002\u9499\u632f\u8361\u662f\u7ec6\u80de\u5185\u9499\u79bb\u5b50\u4ee5\u6d53\u5ea6\u632f\u8361\u53d8\u5316\u7684\u5f62\u5f0f\u4f20\u5bfc\u4fe1\u606f\uff0c\u8fdb\u800c\u8c03\u63a7\u5f71\u54cd\u7ec6\u80de\u6d3b\u52a8\u3002\u5317\u4eac\u5927\u5b66\u674e\u65b9\u5ef7\/\u6b27\u9633\u9880\u56e2\u961f\u5c55\u5f00\u7684\u52a8\u529b\u5b66\u5efa\u6a21\u7814\u7a76\u8868\u660e\uff0c\u80f0\u5c9b\u03b2<\/span><\/strong>\u7ec6\u80de\u5185ATP\u6c34\u5e73\u4e0e\u6c34\u89e3\u81ea\u7531\u80fd\u540c\u65f6\u51b3\u5b9a\u5176\u4e2d\u7684\u9499\u632f\u8361\u3002\u8be5\u5de5\u4f5c\u8fd1\u65e5<\/span><\/strong><\/span><\/strong><\/span><\/strong>\u53d1\u8868\u5728Physical Review Research<\/em>\u671f\u520a\u3002\u5317\u4eac\u5927\u5b66\u7269\u7406\u5b66\u9662\u3001\u5b9a\u91cf\u751f\u7269\u5b66\u4e2d\u5fc3\u674e\u65b9\u5ef7\u526f\u6559\u6388\u4e3a\u8bba\u6587\u7684\u901a\u8baf\u4f5c\u8005\u3002\u5317\u4eac\u5927\u5b66\u7269\u7406\u5b66\u9662\u5b59\u8fd0\u751f\u548c\u674e\u5178\u6770\u4e3a\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002\u8be5\u7814\u7a76\u7684\u6a21\u578b\u6784\u5efa\u4e0e\u5206\u6790\u65b9\u6cd5\u6709\u52a9\u4e8e\u672a\u6765\u8fdb\u4e00\u6b65\u7814\u7a76\u5176\u4ed6\u79bb\u5b50\u901a\u9053\u7cfb\u7edf\u7684\u70ed\u529b\u5b66\u7279\u6027\u3002<\/span><\/strong><\/span><\/strong><\/p>\n<\/section>\n

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\u5173\u952e\u8bcd\uff1aATP\u6c34\u89e3\u53cd\u5e94\uff0c\u7ec6\u80deATP\u6c34\u5e73\uff0c\u52a8\u529b\u5b66\u6a21\u578b\uff0c\u9499\u632f\u8361\u7cfb\u7edf\uff0c\u5206\u53c9<\/strong><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/strong><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/strong><\/strong><\/strong><\/span><\/strong><\/span><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe2 \u5728\u4e0d\u540c\u7684\u7ec6\u80de\u5185ATP \u6d53\u5ea6\uff08[ATP]\uff09\u548cATP \u6c34\u89e3\u81ea\u7531\u80fd\u6c34\u5e73\uff08\u03b3\uff09\u6761\u4ef6\u4e0b\u7684\u9499\u632f\u8361\u72b6\u6001\u3002<\/span><\/p>\n


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\u5728\u672c\u8bba\u6587\u7684\u7b2c\u4e00\u90e8\u5206\u4e2d\uff0c\u7814\u7a76\u8005\u5229\u7528\u4e24\u53d8\u91cf\u6a21\u578b\uff0c\u8ba8\u8bba\u4e86\u9499\u632f\u8361\u662f\u5426\u53d1\u751f\u4f9d\u8d56\u4e8e\u8f93\u5165\u7684[ATP]\u4ee5\u53ca\u6c34\u89e3\u81ea\u7531\u80fd\u3002\u8fd9\u4e00\u90e8\u5206\u6240\u8ba8\u8bba\u7684\u9499\u632f\u8361\u7686\u4e3a\u5468\u671f\u4e3a\u51e0\u79d2\u7684\u9ad8\u9891\u9499\u632f\u8361\u3002\u672c\u8bba\u6587\u4e2d\u7528\u03b3\u4ee3\u8868\u6c34\u89e3\u81ea\u7531\u80fd\u6c34\u5e73\uff0c\u4e00\u6469\u5c14ATP\u6c34\u89e3\u91ca\u653e\u7684\u80fd\u91cf\u4e3a\u0394G=RT In\u03b3\uff0c\u03b3=[ATP]\/(K<\/span>eq<\/sub>[ADP][Pi])<\/span>\uff0c<\/span><\/span>Keq<\/sub>\u4e3aATP\u6c34\u89e3\u53cd\u5e94\u7684\u5e73\u8861\u5e38\u6570\u3002\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u901a\u8fc7\u6539\u53d8\u53c2\u6570[ATP]\u548c\u03b3\u53ef\u4ee5\u4f7f\u9499\u632f\u8361\u7cfb\u7edf\u4ece\u7a33\u5b9a\u6001\u8f6c\u53d8\u4e3a\u632f\u8361\u6001\u3002\u7528\u975e\u7ebf\u6027\u7269\u7406\u5b66\u7684\u8bed\u8a00\u63cf\u8ff0\u5c31\u662f\u8bf4\u7cfb\u7edf\u53d1\u751f\u4e86\u76f8\u53d8\u6216\u8005\u201c\u5206\u53c9\u201d\u3002\u56fe2b-c\u5c55\u793a\u4e86\u4e8c\u7ef4\u7684[ATP]\u548c\u03b3\u7684\u53c2\u6570\u7a7a\u95f4\u4e2d\uff0c\u7cfb\u7edf\u8fbe\u5230\u7a33\u5b9a\u540e\uff0c\u9499\u632f\u8361\u7684\u632f\u5e45\u4e0e\u9891\u7387\uff1b\u5176\u4e2d\u5355\u4e00\u7684\u6df1\u84dd\u7684\u7684\u533a\u57df\u662f\u7a33\u5b9a\u6001\u533a\u57df\uff0c\u4e5f\u5c31\u662f\u6ca1\u6709\u632f\u8361\u4ea7\u751f\u7684\u533a\u57df\uff1b\u800c\u5176\u4ed6\u7684\u6709\u989c\u8272\u7684\u533a\u57df\u5219\u8868\u793a\u5b58\u5728\u9499\u632f\u8361\u7684\u533a\u57df\u3002\u5176\u632f\u5e45\u6216\u9891\u7387\u7684\u6570\u503c\u5206\u522b\u7531\u5176\u989c\u8272\u8868\u793a\u3002\u5c31\u632f\u5e45\u800c\u8a00\uff0c\u632f\u8361\u533a\u7684\u632f\u5e45\u51e0\u4e4e\u7ef4\u6301\u4e0d\u53d8\uff0c\u7136\u800c\u632f\u8361\u533a\u7684\u9891\u7387\u5728\u8fb9\u754c\u5904\u4f1a\u6709\u660e\u663e\u7684\u4e0b\u964d\u3002\u800c\u632f\u8361\u533a\u548c\u975e\u632f\u8361\u533a\u4e24\u4e2a\u533a\u57df\u7684\u8fb9\u754c\u5c31\u662f\u4e4b\u524d\u6240\u8bf4\u7684 \u201c\u5206\u53c9\u201d\u53d1\u751f\u7684\u5730\u65b9\u3002<\/span><\/section>\n

<\/span><\/section>\n
\u7814\u7a76\u8005\u53c8\u5206\u522b\u8003\u5bdf\u4e86[ATP]\u548c\u03b3\u5728\u9499\u632f\u8361\u5206\u53c9\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u3002\u5bf9\u4e8e[ATP]\u6765\u8bf4\uff0c\u5728\u4fdd\u6301\u4e0d\u53d8\u7684\u60c5\u51b5\u4e0b\uff0c\u7cfb\u7edf\u4f1a\u5728\u4e00\u5b9a\u8303\u56f4\u5185\u7684[ATP]\u53c2\u6570\u4e0b\u53d1\u751f\u632f\u8361\u3002\u5728[ATP]\u7531\u4f4e\u6d53\u5ea6\u9010\u6e10\u589e\u52a0\u7684\u8fc7\u7a0b\u4e2d\uff0c\u9996\u5148ATP\u4f7fATP\u8c03\u63a7\u7684\u94be\u79bb\u5b50\u901a\u9053KATP<\/sub>\u5173\u95ed\uff0c\u5bfc\u81f4\u819c\u7535\u4f4d\u7684\u53bb\u6781\u5316\uff0c\u4f7f\u5f97\u9499\u632f\u8361\u4ea7\u751f\u3002\u4f46\u662f\u5f53ATP\u6d53\u5ea6\u8fc7\u9ad8\u4e4b\u540e\uff0c\u819c\u7535\u4f4d\u65e0\u6cd5\u6062\u590d\uff0c\u56e0\u6b64\u632f\u8361\u4fbf\u65e0\u6cd5\u7ef4\u6301\u3002\u63a5\u4e0b\u6765\u8003\u5bdf\u03b3\u7684\u5f71\u54cd\u3002\u5728\u4e0d\u540c\u7684\u03b3\u7684\u5f71\u54cd\u4e0b\uff0c\u5206\u53c9\u70b9\u7684\u4f4d\u7f6e\u4e5f\u4f1a\u53d1\u751f\u6539\u53d8\u3002\u5728\u56fe2(b-c)\u4e2d\u6807\u6ce8\u7684Case 3-I \u548cCase 3-II\uff0c\u5206\u53c9\u8fb9\u754c\u5206\u522b\u5411\u4e24\u4e2a\u4e0d\u540c\u7684\u65b9\u5411\u5f2f\u66f2\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u5206\u53c9\u70b9\u5173\u4e8e\u03b3\u7684\u5173\u7cfb\u4e5f\u662f\u4e0d\u540c\u7684\u3002<\/span><\/section>\n

<\/section>\n
\u4e3a\u4e86\u8fdb\u4e00\u6b65\u89e3\u91ca\u5206\u53c9\u8fb9\u754c\u7684\u5f2f\u66f2\uff0c\u7814\u7a76\u8005\u5bf9\u79bb\u5b50\u901a\u9053\u6a21\u578b\u8fdb\u884c\u4e86\u8fdb\u4e00\u6b65\u7684\u91cf\u5316\u5206\u6790\u3002<\/span>
<\/section>\n
<\/span>
<\/section>\n
<\/section>\n

\u56fe3 \u79bb\u5b50\u901a\u9053\u53d7[ATP]\u548c\u03b3\u7684\u5f71\u54cd\u3002\uff08a-c)[ATP]\u548c\u03b3 \u4f5c\u7528\u4e8eKATP\u901a\u9053\u4e4b\u4e0a\uff0c\u4ea7\u751f\u4e86\u5f2f\u66f2\u7684\u5206\u53c9\u8fb9\u754c\u3002(d-f)PMCA\u4f5c\u4e3a\u6d88\u8017ATP\u6c34\u89e3\u81ea\u7531\u80fd\u7684\u901a\u9053\uff0c\u51b3\u5b9a\u4e86\u5206\u53c9\u8fb9\u754c\u7684\u4e0b\u8fb9\u754c\u3002<\/span><\/p>\n


<\/span><\/section>\n
\u5728Case 3-I\u548cCase 3-II\uff0c\u4e5f\u5c31\u662f\u6ee1\u8db3\u03b32<\/sub> (\u2248 107<\/sup>)< \u03b3 < \u03b31<\/sub>(\u2248 1011<\/sup>)\u7684\u4f4d\u7f6e\uff0c\u5206\u53c9\u70b9\u968f\u03b3\u53d8\u5316\u7684\u5173\u7cfb\u5e76\u4e0d\u76f8\u540c\u3002\u5206\u53c9\u8fb9\u754c\u7684\u8fd9\u79cd\u5f2f\u66f2\u4e8b\u5b9e\u4e0a\u6839\u6e90\u662fKATP<\/sub>\u7684\u5f00\u653e\u6982\u7387\u53d7\u03b3\u7684\u5f71\u54cd\u7684\u6539\u53d8\u3002\u8fd9\u79cd\u5f00\u653e\u6982\u7387\u5728\u6a21\u578b\u5f53\u4e2d\u88ab\u91cf\u5316\u4e3a\u03b8\u3002\u56fe3(a) \u5c55\u793a\u4e86\u03b8\u53d7\u03b3\u548c\u4e0d\u540cATP\u6d53\u5ea6\u7684\u8c03\u63a7\u3002\u5728\u03b3\u7684\u751f\u7406\u6570\u503c\u9644\u8fd1(\u03b3\u22481010<\/sup>) \uff0cKATP<\/sub>\u901a\u9053\u7684\u5f00\u653e\u6982\u7387\u88ab\u5927\u5e45\u63d0\u9ad8\u4e86\u3002\u8fd9\u5728\u56fe3(b)\u5f53\u4e2d\u5f97\u5230\u4e86\u8fdb\u4e00\u6b65\u8bc1\u660e\u3002\u8be5\u56fe\u5c55\u793a\u4e86\u901a\u9053\u5f00\u653e\u6982\u7387\u03b8\u5728[ATP]\u548c\u7684\u4e8c\u7ef4\u53c2\u6570\u7a7a\u95f4\u7684\u5927\u5c0f\u53d8\u5316\u3002\u56e0\u4e3a\u5728\u751f\u7406\u6d53\u5ea6\u9644\u8fd1KATP<\/sub>\u901a\u9053\u7684\u5f00\u653e\u6982\u7387\u589e\u52a0\uff0c\u56e0\u6b64\u9700\u8981\u66f4\u9ad8\u7684ATP\u6d53\u5ea6\u5c06\u8fd9\u4e2a\u901a\u9053\u5173\u95ed\u6389\u3002\u5728Case-3 I\u5f53\u4e2d\u548cCase-3 II\u5f53\u4e2d\uff0c\u5206\u53c9\u8fb9\u754c\u7684\u5f2f\u66f2\u65b9\u5411\u548cKATP<\/sub>\u7684\u5f00\u653e\u6982\u7387\u5728\u8fd9\u4e9b\u533a\u57df\u5185\u7684\u53d8\u5316\u8d8b\u52bf\u662f\u543b\u5408\u7684\u3002\u8fd9\u4e24\u4e2a\u533a\u57df\u5f53\u4e2d\uff0c\u5206\u53c9\u8fb9\u754c\u7684\u5f2f\u66f2\u90fd\u662f\u51fa\u81ea\u540c\u6837\u7684\u539f\u56e0\u3002\u7136\u800c\u5176\u5f15\u8d77\u7684\u6548\u679c\u5374\u662f\u76f8\u53cd\u7684\u3002\u5728Case-3 I\u5f53\u4e2d\uff0c\u66f4\u4f4e\u7684\u80fd\u91cf\u4f7f\u5f97\u89e6\u53d1\u9499\u632f\u8361\u53d8\u5f97\u66f4\u52a0\u56f0\u96be\uff0c\u7136\u800c\u5728Case-3 II\u5f53\u4e2d\u7ed3\u679c\u53cd\u4e86\u8fc7\u6765\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u751f\u7406\u6761\u4ef6\u4e0b\u7684\u503c\u662f\u843d\u5728Case-3 I\u5f53\u4e2d\u3002\u56e0\u6b64\u6709\u7406\u7531\u76f8\u4fe1Case-3 I\u4ee3\u8868\u7684\u60c5\u51b5\uff0c\u4e5f\u5c31\u662f\u66f4\u4f4e\u7684\u80fd\u91cf\u9020\u6210\u9499\u632f\u8361\u53d1\u751f\u56f0\u96be\u7684\u60c5\u51b5\uff0c\u662f\u7b26\u5408\u751f\u7406\u6761\u4ef6\u7684\u60c5\u51b5\u3002<\/span><\/section>\n

<\/span><\/section>\n
\u7814\u7a76\u8005\u7ee7\u7eed\u8003\u5bdf\u4e86\u7ec6\u80de\u819c\u4e0a\u7684\u9499\u79bb\u5b50\u6cf5PMCA\u662f\u5982\u4f55\u6d88\u8017\u81ea\u7531\u80fd\u4ee5\u8fdb\u884c\u9006\u6d53\u5ea6\u68af\u5ea6\u7684\u4e3b\u52a8\u8fd0\u8f93\u7684\u3002\u901a\u8fc7\u8ba1\u7b97\u53ef\u4ee5\u83b7\u77e5\uff0c\u5728\u6ee1\u8db3\u03b3 >\u03b33<\/sub> \u2248 108<\/sup>\u7684\u8303\u56f4\u5185\uff0cPMCA\u51e0\u4e4e\u4e0d\u53d7\u5230\u7684\u5f71\u54cd\u3002\u56e0\u4e3a\u5728\u8fd9\u4e2a\u8303\u56f4\u4e4b\u5185\u5bf9\u4e8ePMCA\u6765\u8bf4\uff0c\u4e0eATP\u6c34\u89e3\u76f8\u8026\u5408\u7684\u53cd\u5e94\u51e0\u4e4e\u662f\u4e0d\u53ef\u9006\u7684\u3002\u7136\u800c\uff0c\u5f53\u03b3\u5c0f\u4e8e\u03b33<\/sub>\u4ee5\u540e\uff0cATP\u6c34\u89e3\u81ea\u7531\u80fd\u5bf9PMCA\u7684\u5f71\u54cd\u6548\u679c\u5f00\u59cb\u53d8\u5f97\u5341\u5206\u663e\u8457\u4e86\u3002JPMCA<\/sub>\u548c\u81ea\u7531\u80fd\u7684\u5173\u7cfb\u5c55\u793a\u5728\u56fe3 (e)\u5f53\u4e2d\u3002\u5176\u4e2dJPMCA<\/sub>\u8868\u5f81\u901a\u8fc7PMCA\u7684\u9499\u79bb\u5b50\u6d41\u3002\u53ef\u4ee5\u770b\u5230\u5728\u8f83\u9ad8\u7684\u60c5\u51b5\u4e0bJPMCA<\/sub>\u786e\u5b9e\u51e0\u4e4e\u4e0d\u53d7\u7684\u5f71\u54cd\uff0c\u800c\u5728\u03b3 < \u03b33<\/sub> \u2248 108<\/sup>\u7684\u8303\u56f4\u5185\uff0cPMCA\u7684\u529f\u80fd\u53d7\u81ea\u7531\u80fd\u7684\u5f71\u54cd\u975e\u5e38\u5927\u3002\u8fd9\u91cc\u9762\uff0cPMCA\u7684\u53cd\u5e94\u9700\u8981\u88ab\u770b\u6210\u662f\u53ef\u9006\u53cd\u5e94\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u8fd8\u5b58\u5728\u4e00\u4e2a\u6700\u5c0f\u7684\u503c\uff0c\u03b34<\/sub> \u2248107<\/sup>\u3002\u8fd9\u4e2a\u503c\u662f\u9499\u632f\u8361\u4ea7\u751f\u7684\u6700\u5c0f\u81ea\u7531\u80fd\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u5982\u679c\u81ea\u7531\u80fd\u4f4e\u4e8e\u03b34<\/sub>\u90a3\u4e48\u9499\u632f\u8361\u5c31\u4e0d\u4f1a\u4ea7\u751f\u3002\u53ef\u4ee5\u901a\u8fc7\u7406\u8bba\u8ba1\u7b97\u7684\u65b9\u6cd5\u53bb\u4f30\u7b97\u03b34<\/sub>\u7684\u91cf\u7ea7\u3002\u7406\u8bba\u8ba1\u7b97\u5f97\u5230\u7684\u6700\u5c0f\u503c\u548c\u6a21\u578b\u6a21\u62df\u5f97\u5230\u7684\u6700\u5c0f\u503c\u57fa\u672c\u4e00\u81f4\u3002<\/span><\/section>\n
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<\/section>\n

<\/p>\n

\u56fe4 \u4e0d\u540c\u7cd6\u6d53\u5ea6\u4e0b\u8bf1\u5bfc\u7684\u9499\u79bb\u5b50\u632f\u8361\u3002(a-b)\u4e09\u79cd\u9499\u79bb\u5b50\u632f\u8361\u6a21\u5f0f\uff08Type\uff09\u548c\u5bf9\u5e94[ATP]\u54cd\u5e94\u3002(c) \u5b9e\u9a8c\u4e2d\u88ab\u89c2\u6d4b\u5230\u6a21\u578b\u9884\u8a00\u7684Type2\u9499\u632f\u8361\u884c\u4e3a\u3002\uff08d\uff09\u4e0d\u540c[ATP]\u4e0e\u03b3\u8f68\u8ff9\u8bf1\u5bfc\u4e0b\u6e38\u7684\u9ad8\u9891\u9499\u632f\u8361\uff08\u6a59\u8272\uff09\uff0c\u7070\u8272\u4e3a\u4e24\u53d8\u91cf\u6a21\u578b\u9884\u6d4b\u7684\u9ad8\u9891\u632f\u8361\u533a\u3002<\/span><\/p>\n


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\u5728\u672c\u8bba\u6587\u7684\u7b2c\u4e8c\u90e8\u5206\u4e2d\uff0c\u4e3a\u4e86\u7406\u89e3\u80f0\u5c9b\u03b2\u7ec6\u80de\u5982\u4f55\u54cd\u5e94\u4e0d\u540c\u7cd6\u6d53\u5ea6\u523a\u6fc0\uff0c\u7814\u7a76\u8005\u7ed3\u5408\u4e24\u53d8\u91cf\u6a21\u578b\u4e0e\u7cd6\u9175\u89e3\u8fc7\u7a0b<\/span>\uff08\u56fe1\uff09<\/span>\uff0c\u6784\u5efa\u4e86\u7cd6\u9175\u89e3-\u9499\u4fe1\u53f7\u6a21\u578b\u3002\u901a\u8fc7\u6570\u503c\u6a21\u62df<\/span>\uff08\u56fe4\uff09<\/span>\uff0c\u53d1\u73b0\u4e86\u4e09\u79cd\u9499\u4fe1\u53f7\u632f\u8361\u6a21\u5f0f<\/span>\uff08Type\uff09<\/span>\uff1aType 1\u548cType 2\u4e2d\uff0c\u5b58\u5728\u5468\u671f\u4e3a\u51e0\u5206\u949f\u7684[ATP]\u6162\u632f\u8361\uff0cATP\u5e26\u52a8\u4e0b\u6e38\u9499\u79bb\u5b50\u6d53\u5ea6\u8fdb\u884c\u6162\u632f\u8361\uff1b\u540c\u65f6\uff0cType 1\u5728\u5cf0\u503c\u5904\u3001Type 2\u5728\u4e0a\u5347\u548c\u4e0b\u964d\u6cbf\uff0c\u5747\u5b58\u5728\u9ad8\u9891\u9499\u632f\u8361\uff0c\u5468\u671f\u4e3a\u79d2\u91cf\u7ea7\uff1b\u5bf9\u4e8eType 3\uff0c\u5b83\u7684[ATP]\u4fdd\u6301\u7a33\u5b9a\uff0c\u800c\u4e0b\u6e38\u4fdd\u6301\u9ad8\u9891\u9499\u632f\u8361\u3002\u7ed3\u5408\u4e24\u53d8\u91cf\u6a21\u578b\u7684[ATP]-\u03b3\u76f8\u56fe\u7ed3\u679c<\/span>\uff08\u56fe2c\uff09<\/span>\uff0c\u7814\u7a76\u8005\u89e3\u91ca\u4e86\u4e09\u79cd\u9499\u632f\u8361\u4fe1\u53f7\u7684\u4ea7\u751f\u673a\u5236\uff1a\u968f\u7740\u7cd6\u6d53\u5ea6\u5347\u9ad8\uff0c\u5176\u8bf1\u5bfc\u7684[ATP]-\u03b3\u8f68\u8ff9\u4e0e\u9ad8\u9891\u9499\u632f\u8361\u533a\u6709\u66f4\u591a\u91cd\u53e0\uff0c\u91cd\u53e0\u533a\u57df\u6709\u9ad8\u9891\u9499\u632f\u8361\u4ea7\u751f\uff1b[ATP]-\u03b3\u8f68\u8ff9\u6700\u7ec8\u6210\u4e3a\u7a33\u5b9a\u70b9\u843d\u5728\u9ad8\u9891\u632f\u8361\u533a\u57df\u5185<\/span>\uff08\u56fe4d\uff09<\/span>\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u7814\u7a76\u8005\u53d1\u73b0\u7684Type2\u9499\u632f\u8361\uff0c\u5728\u524d\u4eba\u7406\u8bba\u6a21\u578b\u4e2d\u57fa\u672c\u6ca1\u6709\u8ba8\u8bba\uff0c\u800c\u5c0f\u9f20\u5b9e\u9a8c\u4e2d\u5374\u89c2\u6d4b\u5230\u8be5\u632f\u8361\u884c\u4e3a14<\/sup><\/span>\uff08\u56fe4c\uff09<\/span>\u3002<\/span><\/section>\n

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\u8bb8\u591a\u7814\u7a76\u8bc1\u660eKATP<\/sub>\u901a\u9053\u86cb\u767d\u7684\u7a81\u53d8\u4f1a\u9020\u6210\u80f0\u5c9b\u03b2\u7ec6\u80de\u80f0\u5c9b\u7d20\u91ca\u653e\u5f02\u5e38\uff0c\u8fd9\u662f2\u578b\u7cd6\u5c3f\u75c5\u7684\u4e3b\u8981\u75c5\u56e0\u4e4b\u4e0015<\/sup>\uff0c\u7814\u7a76\u8005\u57fa\u4e8e\u7cd6\u9175\u89e3-\u9499\u79bb\u5b50\u6a21\u578b\uff0c\u89e3\u91ca\u4e86KATP<\/sub>\u7a81\u53d8\u5982\u4f55\u5f71\u54cd\u80f0\u5c9b\u03b2\u7ec6\u80de\u5bf9\u4e0d\u540c\u7cd6\u6d53\u5ea6\u523a\u6fc0\u7684\u9499\u54cd\u5e94\u3002Tarasov\u7b49\u4eba\u53d1\u73b0\u90e8\u52062\u578b\u7cd6\u5c3f\u75c5\u60a3\u8005\u5b58\u5728KATP<\/sub>\u901a\u9053\u86cb\u767d\u7684Y356C\u7a81\u53d8\uff0c\u5e76\u4e14\u8be5\u7a81\u53d8\u4f1a\u964d\u4f4eATP\u5173\u95edKATP<\/sub>\u901a\u9053\u7684\u80fd\u529b\uff0c\u8fdb\u4e00\u6b65\u5f71\u54cd\u4e0b\u6e38\u9499\u4fe1\u53f7\u548c\u819c\u7535\u4f4d\u7684\u54cd\u5e94\u5f3a\u5ea616<\/sup>\u3002\u56fe5\u4e2d\uff0c\u7814\u7a76\u8005\u9996\u5148\u62df\u5408\u91ce\u751f\u578b\u548c\u7a81\u53d8\u682aKATP<\/sub>\u901a\u9053\u7684\u6253\u5f00\u6982\u7387\u4e0e[ATP]\u5173\u7cfb\u66f2\u7ebf\uff1b\u8fdb\u4e00\u6b65\u8ba1\u7b97\u53d1\u73b0\uff0c\u7a81\u53d8\u8bf1\u5bfc[ATP]-\u03b3\u7a7a\u95f4\u4e2d\u7684\u9ad8\u9891\u9499\u632f\u8361\u533a\u57df\u5411\u53f3\u4e0a\u79fb\u52a8\uff0c\u4f7f\u5f97\u76f8\u540c\u7cd6\u6d53\u5ea6\u4e0b\uff0c[ATP]-\u03b3\u8f68\u8ff9\u4e0e\u9ad8\u9891\u632f\u8361\u533a\u76f8\u4ea4\u90e8\u5206\u51cf\u5c11\u6216\u5b8c\u5168\u6d88\u5931<\/span>\uff08\u56fe5b\uff09<\/span>\uff1b\u56e0\u6b64\uff0cKATP<\/sub>\u7a81\u53d8\u5bfc\u81f4\u76f8\u540c\u7cd6\u6d53\u5ea6\u523a\u6fc0\u4e0b\uff0c\u9ad8\u9891\u9499\u632f\u8361\u548c\u819c\u7535\u538b\u632f\u8361\u51cf\u5c11\u6216\u6d88\u5931\uff0c\u5373\u5e73\u5747\u9499\u54cd\u5e94\u51cf\u5f31<\/span>\uff08\u56fe5c\u548c5d\uff09<\/span>\uff0c\u53ef\u80fd\u5bfc\u81f4\u4e0b\u6e38\u80f0\u5c9b\u7d20\u91ca\u653e\u5f02\u5e38\u3002<\/span><\/section>\n

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\u56fe5 \u91ce\u751f\u578b\u4e0eSUR1-Y356C\u7a81\u53d8\u7684KATP<\/sub>\u901a\u9053\u3002(a)\u7a81\u53d8\u6291\u5236ATP\u5173\u95edKATP<\/sub>\u901a\u9053\u7684\u80fd\u529b\uff0c\u5b9e\u7ebf\u4e3a\u6a21\u578b\u62df\u5408\uff0c\u70b9\u4e3a\u5b9e\u9a8c\u7ed3\u679c\u3002(b)\u7a81\u53d8\u5bfc\u81f4\u9499\u79bb\u5b50\u9ad8\u9891\u632f\u8361\u533a\u5411\u53f3\u4e0a\u79fb\u52a8\u3002\uff08c-d\uff09\u7a81\u53d8\u5bfc\u81f4\u4e0b\u6e38\u9ad8\u9891\u632f\u8361\u65f6\u95f4\u51cf\u5c11\u4e0e\u5e73\u5747\u819c\u7535\u4f4d\u54cd\u5e94\u51cf\u5f31\u3002<\/span><\/p>\n


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\u7efc\u4e0a\uff0c\u672c\u8bba\u6587\u901a\u8fc7\u5efa\u7acb\u52a8\u529b\u5b66\u6a21\u578b\u7684\u65b9\u5f0f\u7814\u7a76ATP\u6c34\u89e3\u81ea\u7531\u80fd\u5bf9\u9499\u632f\u8361\u7684\u8c03\u63a7\u4f5c\u7528\uff0c\u5229\u7528[ATP]-\u81ea\u7531\u80fd\u76f8\u56fe\u7814\u7a76\u4e86\u80f0\u5c9b\u03b2\u7ec6\u80de\u5728\u4e0d\u540c\u7cd6\u6d53\u5ea6\u523a\u6fc0\u4e0b\u7684\u4e09\u79cd\u9499\u54cd\u5e94\u673a\u5236\uff0c\u5e76\u5206\u6790\u4e862\u578b\u7cd6\u5c3f\u75c5\u4e2dKATP<\/sub>\u901a\u9053\u86cb\u767d\u7a81\u53d8\u5982\u4f55\u5bfc\u81f4\u80f0\u5c9b\u03b2\u7ec6\u80de\u54cd\u5e94\u5f02\u5e38\u3002\u7814\u7a76\u8005\u8ba4\u4e3a\u672c\u8bba\u6587\u7684\u6a21\u578b\u6784\u5efa\u4e0e\u5206\u6790\u65b9\u6cd5\u6709\u52a9\u4e8e\u672a\u6765\u7814\u7a76\u5176\u4ed6\u79bb\u5b50\u901a\u9053\u7cfb\u7edf\u7684\u70ed\u529b\u5b66\u7279\u6027\u3002<\/span><\/section>\n

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\u5728\u5ba1\u7a3f\u8fc7\u7a0b\u4e2d\uff0c\u5ba1\u7a3f\u4eba\u5bf9\u672c\u8bba\u6587\u7ed3\u679c\u63d0\u51fa\u8f83\u9ad8\u8bc4\u4ef7\u3002\u90e8\u5206\u5ba1\u7a3f\u610f\u89c1\u5982\u4e0b\uff1a\u5ba1\u7a3f\u4eba\uff081\uff09\uff1a\u201cI believe that reproducing  the experimental results of WT and the Y356C mutant with a single model is very important achievement. I am sure that this work adds a lot of new knowledge  in the area of Biophysics, since it addresses a problem from various angles (kinetics, thermodynamics & energy).\u201d\u5ba1\u7a3f\u4eba\uff082\uff09\uff1a\u201cThe two-stage analyses provide a clear picture on calcium oscillations and the roles of hydrolysis and glycolysis.\u201d<\/span><\/section>\n

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\u5317\u4eac\u5927\u5b66\u7269\u7406\u5b66\u9662\u3001\u5b9a\u91cf\u751f\u7269\u5b66\u4e2d\u5fc3\u674e\u65b9\u5ef7\u526f\u6559\u6388\u4e3a\u8bba\u6587\u7684\u901a\u8baf\u4f5c\u8005\u3002\u5317\u4eac\u5927\u5b66\u7269\u7406\u5b66\u9662\u5b59\u8fd0\u751f\u548c\u674e\u5178\u6770\u4e3a\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002\u8be5\u7814\u7a76\u5f97\u5230\u4e86\u56fd\u5bb6\u91cd\u70b9\u7814\u53d1\u8ba1\u5212\u3001\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u7b49\u673a\u6784\u7684\u652f\u6301\u3002<\/span><\/section>\n

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1. Hill TL. Free energy transduction and biochemical cycle kinetics<\/em>, 1989 edn. Springer: New York, NY, 2012.<\/span><\/section>\n
2. Qian H. Phosphorylation energy hypothesis: open chemical systems and their biological functions. Annu Rev Phys Chem<\/em> 2007, 58: 113-142.<\/span><\/section>\n
3. Hopfield JJ. Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity. Proc Natl Acad Sci U S A<\/em> 1974, 71(10): 4135-4139.<\/span><\/section>\n
4. Bennett CH. Dissipation-error tradeoff in proofreading. Biosystems<\/em> 1979, 11(2-3): 85-91.<\/span><\/section>\n
5. Tu Y. The nonequilibrium mechanism for ultrasensitivity in a biological switch: sensing by Maxwell’s demons. Proc Natl Acad Sci U S A<\/em> 2008, 105(33): 11737-11741.<\/span><\/section>\n
6. Cao Y, Wang H, Ouyang Q, Tu Y. The free energy cost of accurate biochemical oscillations. Nat Phys<\/em> 2015, 11(9): 772-778.<\/span><\/section>\n
7. Zhang D, Cao Y, Ouyang Q, Tu Y. The energy cost and optimal design for synchronization of coupled molecular oscillators. Nat Phys<\/em> 2020, 16(1): 95-100.<\/span><\/section>\n
8. Yang X, Heinemann M, Howard J, Huber G, Iyer-Biswas S, Le Treut G, et al.<\/em> Physical bioenergetics: Energy fluxes, budgets, and constraints in cells. Proc Natl Acad Sci U S A<\/em> 2021, 118(26): e2026786118.<\/span><\/section>\n
9. Sun Y, Li D, Ni C, Ge Y, Qian H, Ouyang Q, et al.<\/em> ATP hydrolysis kinetics and thermodynamics as determinants of calcium oscillation in pancreatic \u03b2 cells. Phys Rev Research<\/em> 2022, 4(4).<\/span><\/section>\n
10. Wilson DF, Cember ATJ, Matschinsky FM. The thermodynamic basis of glucose-stimulated insulin release: a model of the core mechanism. Physiol Rep<\/em> 2017, 5(12): e13327.<\/span><\/section>\n
11. Trexler AJ, Taraska JW. Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells. Cell Calcium<\/em> 2017, 67: 1-10.<\/span><\/section>\n
12. Barker CJ, Li L, K\u00f6hler M, Berggren P-O. \u03b2-Cell Ca(2+) dynamics and function are compromised in aging. Adv Biol Regul<\/em> 2015, 57: 112-119.<\/span><\/section>\n
13. F\u00e9lix-Mart\u00ednez GJ, God\u00ednez-Fern\u00e1ndez JR. Mathematical models of electrical activity of the pancreatic \u03b2-cell: a physiological review. Islets<\/em> 2014, 6(3): e949195.<\/span><\/section>\n
14. Beauvois MC, Merezak C, Jonas J-C, Ravier MA, Henquin J-C, Gilon P. Glucose-induced mixed [Ca2+]c oscillations in mouse beta-cells are controlled by the membrane potential and the SERCA3 Ca2+-ATPase of the endoplasmic reticulum. Am J Physiol Cell Physiol<\/em> 2006, 290(6): C1503-1511.<\/span><\/section>\n
15. Pipatpolkai T, Usher S, Stansfeld PJ, Ashcroft FM. New insights into KATP channel gene mutations and neonatal diabetes mellitus. Nat Rev Endocrinol<\/em> 2020, 16(7): 378-393.<\/span><\/section>\n
16. Tarasov AI, Nicolson TJ, Riveline J-P, Taneja TK, Baldwin SA, Baldwin JM, et al.<\/em> A rare mutation in ABCC8\/SUR1 leading to altered ATP-sensitive K+ channel activity and beta-cell glucose sensing is associated with type 2 diabetes in adults. Diabetes<\/em> 2008, 57(6): 1595-1604.<\/span><\/section>\n


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\uff08\u53c2\u8003\u6587\u732e\u53ef\u4e0a\u4e0b\u6ed1\u52a8<\/strong>\u67e5\u770b\uff09<\/p>\n<\/section>\n<\/section>\n


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\u590d\u6742\u79d1\u5b66\u6700\u65b0\u8bba\u6587<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u96c6\u667a\u6591\u56fe\u9876\u520a\u8bba\u6587\u901f\u9012\u680f\u76ee\u4e0a\u7ebf\u4ee5\u6765\uff0c\u6301\u7eed\u6536\u5f55\u6765\u81eaNature\u3001Science<\/span>\u7b49\u9876\u520a\u7684\u6700\u65b0\u8bba\u6587\uff0c\u8ffd\u8e2a\u590d\u6742\u7cfb\u7edf\u3001\u7f51\u7edc\u79d1\u5b66\u3001\u8ba1\u7b97\u793e\u4f1a\u79d1\u5b66\u7b49\u9886\u57df\u7684\u524d\u6cbf\u8fdb\u5c55\u3002\u73b0\u5728\u6b63\u5f0f\u63a8\u51fa\u8ba2\u9605\u529f\u80fd\uff0c\u6bcf\u5468\u901a\u8fc7\u5fae\u4fe1\u670d\u52a1\u53f7\u300c\u6211\u7684\u96c6\u667a\u300d\u63a8\u9001\u8bba\u6587\u4fe1\u606f\u3002\u626b\u63cf\u4e0b\u65b9\u4e8c\u7ef4\u7801\u5373\u53ef\u4e00\u952e\u8ba2\u9605\uff1a<\/span>
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1. <\/strong>PRL\u524d\u6cbf\uff1a\u63a2\u7a76\u975e\u5e73\u8861\u6001\u8f93\u8fd0\u4e3b\u5bfc\u8def\u5f84\u65b0\u65b9\u6cd5\u2014\u2014\u5708\u6d41\u6392\u5e8f<\/a><\/strong><\/span><\/section>\n
2. <\/strong>Physics Reports\u901f\u9012\uff1a\u975e\u5e73\u8861\u6001\u7c97\u7c92\u5316\u5efa\u6a21<\/a><\/strong><\/span><\/section>\n
3. <\/strong>\u4ec0\u4e48\u662f\u975e\u5e73\u8861\u6001\u70ed\u529b\u5b66 | \u96c6\u667a\u767e\u79d1<\/a><\/strong><\/span><\/section>\n
4. \u300a\u5f20\u6c5f\u00b7\u590d\u6742\u79d1\u5b66\u524d\u6cbf27\u8bb2\u300b\u5b8c\u6574\u4e0a\u7ebf\uff01<\/strong><\/a><\/strong><\/strong><\/strong><\/section>\n
5. \u6210\u4e3a\u96c6\u667aVIP\uff0c\u89e3\u9501\u5168\u7ad9\u8bfe\u7a0b\uff0f\u8bfb\u4e66\u4f1a<\/span><\/strong><\/a><\/strong><\/strong><\/strong><\/strong><\/section>\n
6. \u52a0\u5165\u96c6\u667a\uff0c\u4e00\u8d77\u590d\u6742\uff01<\/strong><\/a><\/strong><\/strong><\/strong><\/section>\n


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