{"id":51150,"date":"2024-06-30T20:52:17","date_gmt":"2024-06-30T12:52:17","guid":{"rendered":"https:\/\/swarma.org\/?p=51150"},"modified":"2024-06-30T20:52:17","modified_gmt":"2024-06-30T12:52:17","slug":"%e6%95%b4%e5%90%88%e7%94%9f%e5%91%bd%e7%bb%84%e5%ad%a6%e6%95%b0%e6%8d%ae%ef%bc%8c%e6%8f%ad%e7%a4%ba%e7%94%9f%e5%91%bd%e5%a4%8d%e6%9d%82%e7%b3%bb%e7%bb%9f%e6%9e%84%e6%88%90%e5%8e%9f%e7%90%86","status":"publish","type":"post","link":"https:\/\/swarma.org\/?p=51150","title":{"rendered":"\u6574\u5408\u751f\u547d\u7ec4\u5b66\u6570\u636e\uff0c\u63ed\u793a\u751f\u547d\u590d\u6742\u7cfb\u7edf\u6784\u6210\u539f\u7406"},"content":{"rendered":"
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\u6591\u56fe\u5730\u5740\uff1ahttps:\/\/pattern.swarma.org\/articles<\/span><\/span><\/p>\n


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\u8f6c\u5f55\u7ec4\u5b66<\/span><\/strong>\uff08Transcriptomics\uff09\u662f\u7814\u7a76\u7ec6\u80de\u3001\u7ec4\u7ec7\u6216\u751f\u7269\u4f53\u4e2d\u6240\u6709\u8f6c\u5f55\u4ea7\u7269\uff08\u5373RNA\u5206\u5b50\uff09\u7684\u4e00\u95e8\u79d1\u5b66\u3002\u8f6c\u5f55\u7ec4\u5305\u62ec\u4fe1\u4f7fRNA\uff08mRNA\uff09\u3001\u975e\u7f16\u7801RNA\uff08\u5982\u5c0f\u6838RNA\uff08snRNA\uff09\u3001\u5c0f\u6838\u4ec1RNA\uff08snoRNA\uff09\u3001\u957f\u94fe\u975e\u7f16\u7801RNA\uff08lncRNA\uff09\u4ee5\u53ca\u5faeRNA\uff08miRNA\uff09\u7b49\uff09\u3002\u8f6c\u5f55\u7ec4\u5b66\u7684\u4e3b\u8981\u76ee\u7684\u662f\u5168\u9762\u5206\u6790\u548c\u89e3\u91ca\u57fa\u56e0\u8868\u8fbe\u53ca\u5176\u8c03\u63a7\u673a\u5236\u3002<\/span><\/p>\n


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\u4e00\u3001\u8f6c\u5f55\u7ec4\u5b66\u7684\u57fa\u672c\u6982\u5ff5\u4e0e\u6570\u636e\u63cf\u8ff0<\/strong><\/span><\/section>\n
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  1. \n
    \u8f6c\u5f55\u7ec4\uff1a\u6307\u4e00\u4e2a\u7279\u5b9a\u65f6\u95f4\u70b9\u3001\u7279\u5b9a\u6761\u4ef6\u4e0b\uff0c\u67d0\u4e00\u7ec6\u80de\u3001\u7ec4\u7ec7\u6216\u751f\u7269\u4f53\u4e2d\u6240\u6709\u7684RNA\u8f6c\u5f55\u4ea7\u7269\u7684\u96c6\u5408\u3002<\/span><\/section>\n<\/li>\n
  2. \n
    \u8f6c\u5f55\u4ea7\u7269\uff1a\u4e3b\u8981\u5305\u62ecmRNA\u548c\u5404\u79cd\u7c7b\u578b\u7684\u975e\u7f16\u7801RNA\uff0c\u5982rRNA\u3001tRNA\u3001miRNA\u3001lncRNA\u7b49\u3002<\/span><\/section>\n<\/li>\n
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    \u8f6c\u5f55\u7ec4\u6570\u636e\u901a\u5e38\u88ab\u7ec4\u7ec7\u6210\u4e00\u4e2a\u4e8c\u7ef4\u77e9\u9635\uff0c\u6bcf\u4e00\u884c\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u800c\u6bcf\u4e00\u5217\u4ee3\u8868\u4e00\u4e2a\u6837\u672c\uff0c\u8868\u793a\u6bcf\u4e2a\u57fa\u56e0\u5728\u4e0d\u540c\u6837\u672c\u4e2d\u7684\u8868\u8fbe\u6c34\u5e73\uff0c\u53ef\u4ee5\u53cd\u6620\u51fa\u5176\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u6d3b\u8dc3\u7a0b\u5ea6\u3002\u6837\u672c\u53ef\u4ee5\u6765\u81ea\u4e0d\u540c\u4e2a\u4f53\u7684\u751f\u7269\u6837\u672c\uff0c\u4e0d\u540c\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u7684\u6837\u672c\uff0c\u6216\u8005\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u6837\u672c\u7b49\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


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    \u4e8c\u3001\u8f6c\u5f55\u7ec4\u5b66\u7684\u7814\u7a76\u65b9\u6cd5<\/strong><\/span><\/section>\n
    \u5fae\u9635\u5217\u6280\u672f\uff08Microarray\uff09\uff1a<\/strong>\u5fae\u9635\u5217\u6280\u672f\u5229\u7528\u5df2\u77e5\u5e8f\u5217\u7684\u63a2\u9488\u68c0\u6d4b\u6837\u672c\u4e2d\u5bf9\u5e94\u7684mRNA\u6216cDNA\uff0c\u901a\u8fc7\u8367\u5149\u4fe1\u53f7\u7684\u5f3a\u5f31\u53cd\u6620\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002<\/span><\/section>\n
    \u4f18\u70b9\uff1a\u6210\u672c\u4f4e\uff0c\u6570\u636e\u5904\u7406\u76f8\u5bf9\u7b80\u5355\u3002<\/span><\/section>\n

    \u7f3a\u70b9\uff1a\u53ea\u80fd\u68c0\u6d4b\u5df2\u77e5\u5e8f\u5217\uff0c\u7075\u654f\u5ea6\u548c\u52a8\u6001\u8303\u56f4\u6709\u9650\u3002<\/span><\/p>\n


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    RNA\u6d4b\u5e8f\uff08RNA-Seq\uff09\uff1a<\/strong>RNA-Seq\u662f\u57fa\u4e8e\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6280\u672f\uff0c\u901a\u8fc7\u5bf9cDNA\u6587\u5e93\u8fdb\u884c\u6df1\u5ea6\u6d4b\u5e8f\uff0c\u83b7\u53d6\u6837\u672c\u4e2dRNA\u7684\u5e8f\u5217\u4fe1\u606f\u3002<\/span><\/section>\n
    \u4f18\u70b9\uff1a\u7075\u654f\u5ea6\u9ad8\uff0c\u80fd\u68c0\u6d4b\u672a\u77e5\u548c\u4f4e\u4e30\u5ea6\u7684\u8f6c\u5f55\u4ea7\u7269\uff0c\u52a8\u6001\u8303\u56f4\u5927\u3002<\/span><\/section>\n

    \u7f3a\u70b9\uff1a\u6210\u672c\u8f83\u9ad8\uff0c\u6570\u636e\u5904\u7406\u590d\u6742\u3002<\/span><\/p>\n


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      \n
    1. \n
      \u57fa\u56e0\u8868\u8fbe\u8c31\u5206\u6790\uff1a\u901a\u8fc7\u6bd4\u8f83\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\uff0c\u8bc6\u522b\u4e0e\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b\u6216\u75be\u75c5\u76f8\u5173\u7684\u57fa\u56e0\u3002\u5e94\u7528\u4e8e\u764c\u75c7\u3001\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u3001\u611f\u67d3\u6027\u75be\u75c5\u7b49\u7814\u7a76\u4e2d\u3002<\/span><\/section>\n<\/li>\n
    2. \n
      \u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\u6784\u5efa\uff1a\u7814\u7a76\u57fa\u56e0\u8868\u8fbe\u7684\u8c03\u63a7\u673a\u5236\uff0c\u6784\u5efa\u57fa\u56e0\u8c03\u63a7\u7f51\u7edc\uff0c\u63ed\u793a\u57fa\u56e0\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u548c\u8c03\u63a7\u5173\u7cfb\u3002\u6709\u52a9\u4e8e\u7406\u89e3\u590d\u6742\u7684\u751f\u7269\u5b66\u8fc7\u7a0b\uff0c\u5982\u53d1\u80b2\u3001\u7ec6\u80de\u5206\u5316\u3001\u5e94\u6fc0\u53cd\u5e94\u7b49\u3002<\/span><\/section>\n<\/li>\n
    3. \n
      \u65b0\u8f6c\u5f55\u672c\u7684\u53d1\u73b0\uff1aRNA-Seq\u6280\u672f\u53ef\u4ee5\u53d1\u73b0\u65b0\u7684\u8f6c\u5f55\u672c\u548c\u53ef\u53d8\u526a\u63a5\u4e8b\u4ef6\uff0c\u63ed\u793a\u57fa\u56e0\u8868\u8fbe\u7684\u590d\u6742\u6027\u548c\u591a\u6837\u6027\u3002\u5e2e\u52a9\u5b8c\u5584\u57fa\u56e0\u7ec4\u6ce8\u91ca\uff0c\u62d3\u5c55\u5bf9\u57fa\u56e0\u529f\u80fd\u7684\u8ba4\u8bc6\u3002<\/span><\/section>\n<\/li>\n
    4. \n
      \u975e\u7f16\u7801RNA\u7684\u7814\u7a76\uff1a\u975e\u7f16\u7801RNA\u5728\u57fa\u56e0\u8c03\u63a7\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u5982miRNA\u53c2\u4e0e\u57fa\u56e0\u6c89\u9ed8\uff0clncRNA\u53c2\u4e0e\u67d3\u8272\u8d28\u4fee\u9970\u548c\u8f6c\u5f55\u8c03\u63a7\u3002\u7814\u7a76\u975e\u7f16\u7801RNA\u7684\u529f\u80fd\u548c\u673a\u5236\uff0c\u63ed\u793a\u5176\u5728\u53d1\u80b2\u548c\u75be\u75c5\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


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      \u56db\u3001\u8f6c\u5f55\u7ec4\u6570\u636e\u5206\u6790<\/strong><\/span><\/section>\n
      1. \u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff08Differential Expression Analysis\uff09\uff1a\u901a\u8fc7\u7edf\u8ba1\u65b9\u6cd5\uff08\u5982DESeq2, edgeR\uff09\u8bc6\u522b\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002<\/span><\/section>\n

      \u4f8b\u5b50\uff1a\u5bf9\u5b9e\u9a8c\u7ec4\u548c\u5bf9\u7167\u7ec4\u7684\u57fa\u56e0\u8868\u8fbe\u505a\u5dee\u5f02\u5206\u6790\uff0c\u627e\u51fa\u76f8\u5bf9\u4e8e\u5bf9\u7167\u7ec4\u4e2d\uff0c\u5b9e\u9a8c\u7ec4\u7684\u4e0a\u4e0b\u8c03\u57fa\u56e0\uff1a<\/span><\/p>\n


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      <\/span><\/p>\n

      2. \u529f\u80fd\u5bcc\u96c6\u5206\u6790\uff08Functional Enrichment Analysis\uff09\uff1a\u5bf9\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u8fdb\u884c\u57fa\u56e0\u672c\u4f53\uff08GO\uff09\u5bcc\u96c6\u5206\u6790\u3001\u901a\u8def\u5206\u6790\uff08\u5982KEGG\uff09\u3001GSEA\uff08\u57fa\u56e0\u96c6\u5bcc\u96c6\u5206\u6790\uff09\u7b49\u3002<\/span><\/p>\n


      <\/span><\/p>\n

      <\/p>\n

      <\/p>\n


      <\/span><\/p>\n

      3. \u5171\u8868\u8fbe\u7f51\u7edc\u5206\u6790\uff08Co-expression Network Analysis\uff09\uff1a\u6784\u5efa\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\uff0c\u8bc6\u522b\u529f\u80fd\u6a21\u5757\uff08\u5982WGCNA\uff09\uff0c\u5206\u6790\u6a21\u5757\u4e0e\u8868\u578b\u7684\u5173\u7cfb\u3002<\/span><\/section>\n

      \u4f8b\u5b50\uff1a\u901a\u8fc7\u5bf9\u52a8\u6001\u5207\u5272\u805a\u7c7b\uff0c\u627e\u5230\u7a33\u5065\u7684\u57fa\u56e0\u6a21\u5757\uff0c\u5e76\u5c06\u6a21\u5757\u7684\u7279\u5f81\u57fa\u56e0\u548c\u4e34\u5e8a\u6027\u72b6\u505a\u5173\u8054\uff0c\u4ee5\u6b64\u6765\u627e\u5230\u548c\u76ee\u6807\u4e34\u5e8a\u5f62\u72b6\u5173\u8054\u7684\u57fa\u56e0\u6a21\u5757\u3002<\/span><\/p>\n


      <\/span><\/p>\n

      <\/p>\n


      <\/span><\/p>\n

      4. \u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u4f4d\u70b9\u5206\u6790\uff08Transcription Factor Binding Site Analysis\uff09\uff1a\u901a\u8fc7Motif\u5206\u6790\u8bc6\u522b\u8f6c\u5f55\u56e0\u5b50\u7684\u6f5c\u5728\u7ed3\u5408\u4f4d\u70b9\uff0c\u9884\u6d4b\u8c03\u63a7\u7f51\u7edc\u3002<\/span><\/section>\n

      \u4f8b\u5b50\uff1a\u901a\u8fc7\u7edf\u8ba1\u6bcf\u4e2a\u78b1\u57fa\u5728\u4e0d\u540c\u4f4d\u7f6e\u7684\u9891\u7387\uff08PFM\uff09\uff0c\u8f6c\u6362\u4e3a\u8bc4\u5206\u77e9\u9635\uff08PSSM\uff09\uff0c\u5e76\u4f7f\u7528\u8fd9\u4e9b\u8bc4\u5206\u77e9\u9635\u8ba1\u7b97\u65b0\u7684DNA\u5e8f\u5217\u4e0eSPI1\u7ed3\u5408\u4f4d\u70b9\u7684\u5339\u914d\u5206\u6570\u3002\u8fd9\u4e00\u8fc7\u7a0b\u5e2e\u52a9\u8bc6\u522b\u54ea\u4e9bDNA\u5e8f\u5217\u53ef\u80fd\u662f\u8f6c\u5f55\u56e0\u5b50\u7684\u7ed3\u5408\u4f4d\u70b9\u3002<\/span><\/p>\n


      <\/span><\/p>\n

      <\/p>\n


      <\/span><\/p>\n

      5. \u53ef\u53d8\u526a\u63a5\u5206\u6790\uff08Alternative Splicing Analysis\uff09\uff1a\u901a\u8fc7\u8f6f\u4ef6\u5982rMATS, MISO\u5206\u6790\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u53ef\u53d8\u526a\u63a5\u4e8b\u4ef6\u3002<\/span><\/p>\n


      <\/p>\n


      <\/p>\n

      \n
      \n
      \n
      \n
      \n

      <\/section>\n

      <\/section>\n
      \n
      <\/svg><\/section>\n<\/section>\n<\/section>\n
      \n

      \u86cb\u767d\u8d28\u7ec4\u5b66<\/strong><\/span><\/strong><\/strong><\/p>\n<\/section>\n

      \n

      <\/section>\n

      <\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/h3>\n


      <\/span><\/p>\n

      \u86cb\u767d\u8d28\u7ec4\u5b66<\/span><\/strong>\uff08Proteomics\uff09\u662f\u7814\u7a76\u7ec6\u80de\u3001\u7ec4\u7ec7\u6216\u751f\u7269\u4f53\u5185\u6240\u6709\u86cb\u767d\u8d28\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\u7684\u4e00\u95e8\u79d1\u5b66\u3002\u86cb\u767d\u8d28\u662f\u751f\u547d\u6d3b\u52a8\u7684\u76f4\u63a5\u6267\u884c\u8005\uff0c\u7814\u7a76\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u3001\u4fee\u9970\u3001\u76f8\u4e92\u4f5c\u7528\u53ca\u5176\u5728\u751f\u7269\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\uff0c\u5bf9\u4e8e\u7406\u89e3\u751f\u547d\u73b0\u8c61\u548c\u75be\u75c5\u673a\u5236\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u4ee5\u4e0b\u662f\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u8be6\u7ec6\u4ecb\u7ecd\uff1a<\/span><\/p>\n


      <\/p>\n

      \u4e00\u3001\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u57fa\u672c\u6982\u5ff5\u4e0e\u6570\u636e\u63cf\u8ff0<\/strong><\/span><\/p>\n


      <\/span><\/p>\n

      \u6307\u5728\u7279\u5b9a\u65f6\u95f4\u3001\u7279\u5b9a\u6761\u4ef6\u4e0b\uff0c\u67d0\u4e00\u7ec6\u80de\u3001\u7ec4\u7ec7\u6216\u751f\u7269\u4f53\u4e2d\u6240\u6709\u86cb\u767d\u8d28\u7684\u96c6\u5408\u3002\u65e8\u5728\u5168\u9762\u5206\u6790\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u6c34\u5e73\u3001\u4fee\u9970\u72b6\u6001\u3001\u76f8\u4e92\u4f5c\u7528\u548c\u529f\u80fd\u3002<\/span><\/p>\n


      <\/p>\n

      \u86cb\u767d\u8d28\u7ec4\u6570\u636e\u901a\u5e38\u4e5f\u88ab\u7ec4\u7ec7\u6210\u4e00\u4e2a\u4e8c\u7ef4\u77e9\u9635\uff0c\u6bcf\u4e00\u884c\u4ee3\u8868\u4e00\u4e2a\u86cb\u767d\u8d28\uff0c\u6bcf\u4e00\u5217\u4ee3\u8868\u4e00\u4e2a\u6837\u672c\uff0c\u8868\u793a\u6bcf\u4e2a\u86cb\u767d\u8d28\u5728\u4e0d\u540c\u6837\u672c\u4e2d\u7684\u4e30\u5ea6\uff0c\u53ef\u4ee5\u53cd\u6620\u5176\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u529f\u80fd\u548c\u6d3b\u6027\u3002\u6837\u672c\u53ef\u4ee5\u662f\u6765\u81ea\u4e0d\u540c\u4e2a\u4f53\u7684\u751f\u7269\u6837\u672c\uff0c\u4e0d\u540c\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u7684\u6837\u672c\uff0c\u6216\u8005\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u6837\u672c\u7b49\u3002<\/span><\/p>\n


      <\/p>\n

      \u4e8c\u3001\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u7814\u7a76\u65b9\u6cd5<\/strong><\/span><\/p>\n


      <\/strong><\/span><\/p>\n

      \u53cc\u5411\u7535\u6cf3\uff082-DE\uff09<\/strong>\uff1a\u901a\u8fc7\u7b49\u7535\u805a\u7126\uff08IEF\uff09\u548cSDS-PAGE\u5206\u79bb\u86cb\u767d\u8d28\uff0c\u6839\u636e\u86cb\u767d\u8d28\u7684\u7b49\u7535\u70b9\u548c\u5206\u5b50\u91cf\u8fdb\u884c\u5206\u6790\u3002<\/span><\/p>\n

      \u4f18\u70b9\uff1a\u5206\u8fa8\u7387\u9ad8\uff0c\u9002\u7528\u4e8e\u590d\u6742\u6837\u54c1\u3002<\/span><\/p>\n

      \u7f3a\u70b9\uff1a\u64cd\u4f5c\u590d\u6742\uff0c\u65e0\u6cd5\u68c0\u6d4b\u4f4e\u4e30\u5ea6\u6216\u96be\u6eb6\u7684\u86cb\u767d\u8d28\u3002<\/span><\/p>\n


      <\/p>\n

      \u8d28\u8c31\u5206\u6790\uff08Mass Spectrometry, MS\uff09<\/strong>\uff1a\u8d28\u8c31\u6280\u672f\u901a\u8fc7\u79bb\u5b50\u5316\u86cb\u767d\u8d28\u6216\u80bd\u6bb5\uff0c\u6d4b\u91cf\u5176\u8d28\u8377\u6bd4\uff08m\/z\uff09\uff0c\u7528\u4e8e\u86cb\u767d\u8d28\u9274\u5b9a\u548c\u5b9a\u91cf\u3002<\/span><\/p>\n

      \u4f18\u70b9\uff1a\u7075\u654f\u5ea6\u9ad8\uff0c\u9002\u7528\u4e8e\u9ad8\u901a\u91cf\u5206\u6790\u3002<\/span><\/p>\n

      \u7f3a\u70b9\uff1a\u9700\u8981\u590d\u6742\u7684\u6570\u636e\u5206\u6790\u548c\u89e3\u91ca\u3002<\/span><\/p>\n


      <\/p>\n

      \u86cb\u767d\u8d28\u82af\u7247\uff08Protein Microarray\uff09<\/strong>\uff1a\u5229\u7528\u56fa\u5b9a\u5728\u82af\u7247\u4e0a\u7684\u7279\u5f02\u6027\u6297\u4f53\u6216\u914d\u4f53\uff0c\u68c0\u6d4b\u6837\u54c1\u4e2d\u76f8\u5e94\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u6c34\u5e73\u3002<\/span><\/p>\n

      \u4f18\u70b9\uff1a\u9ad8\u901a\u91cf\uff0c\u9002\u7528\u4e8e\u86cb\u767d\u8d28\u8868\u8fbe\u8c31\u5206\u6790\u3002<\/span><\/p>\n

      \u7f3a\u70b9\uff1a\u4f9d\u8d56\u4e8e\u5df2\u77e5\u86cb\u767d\u8d28\u548c\u6297\u4f53\u7684\u8d28\u91cf\u3002<\/span><\/p>\n


      <\/p>\n

      \u4e09\u3001\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u5e94\u7528<\/strong><\/span><\/section>\n
        \n
      1. \n
        \u86cb\u767d\u8d28\u8868\u8fbe\u8c31\u5206\u6790\uff1a\u901a\u8fc7\u6bd4\u8f83\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u86cb\u767d\u8d28\u8868\u8fbe\u5dee\u5f02\uff0c\u8bc6\u522b\u4e0e\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b\u6216\u75be\u75c5\u76f8\u5173\u7684\u86cb\u767d\u8d28\u3002<\/span><\/section>\n<\/li>\n
      2. \n
        \u86cb\u767d\u8d28\u4fee\u9970\u5206\u6790\uff1a\u7814\u7a76\u86cb\u767d\u8d28\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\uff08\u5982\u78f7\u9178\u5316\u3001\u4e59\u9170\u5316\u3001\u7cd6\u57fa\u5316\u7b49\uff09\uff0c\u63ed\u793a\u5176\u5728\u4fe1\u53f7\u4f20\u5bfc\u548c\u529f\u80fd\u8c03\u63a7\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/section>\n<\/li>\n
      3. \n
        \u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u7814\u7a76\uff1a\u901a\u8fc7\u86cb\u767d\u8d28\u4e92\u4f5c\u7f51\u7edc\uff0c\u5171\u514d\u75ab\u6c89\u6dc0\uff08Co-IP\uff09\u3001\u9175\u6bcd\u53cc\u6742\u4ea4\uff08Y2H\uff09\u3001\u62c9\u66fc\u5149\u8c31\u7b49\u6280\u672f\uff0c\u7814\u7a76\u86cb\u767d\u8d28\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\u3002\u6709\u52a9\u4e8e\u7406\u89e3\u86cb\u767d\u8d28\u5728\u7ec6\u80de\u5185\u7684\u529f\u80fd\u7f51\u7edc\u548c\u8c03\u63a7\u673a\u5236\u3002<\/span><\/section>\n<\/li>\n
      4. \n
        \u751f\u7269\u6807\u5fd7\u7269\u53d1\u73b0\uff1a\u901a\u8fc7\u86cb\u767d\u8d28\u7ec4\u5b66\u7814\u7a76\uff0c\u5bfb\u627e\u75be\u75c5\u7279\u5f02\u6027\u86cb\u767d\u8d28\u4f5c\u4e3a\u751f\u7269\u6807\u5fd7\u7269\uff0c\u7528\u4e8e\u65e9\u671f\u8bca\u65ad\u548c\u9884\u540e\u8bc4\u4f30\u3002\u4f8b\u5982\uff0c\u764c\u75c7\u4e2d\u7684\u7279\u5f02\u6027\u86cb\u767d\u8d28\u6807\u5fd7\u7269\u3002<\/span><\/section>\n<\/li>\n
      5. \n
        \u836f\u7269\u9776\u70b9\u9274\u5b9a\uff1a\u901a\u8fc7\u86cb\u767d\u8d28\u7ec4\u5b66\u7814\u7a76\uff0c\u9274\u5b9a\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u86cb\u767d\u8d28\u4f5c\u4e3a\u6f5c\u5728\u7684\u836f\u7269\u9776\u70b9\uff0c\u63a8\u52a8\u65b0\u836f\u5f00\u53d1\u3002\u4f8b\u5982\uff0c\u9488\u5bf9\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u6291\u5236\u5242\u5f00\u53d1\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


        <\/p>\n

        \u56db\u3001\u86cb\u767d\u8d28\u7ec4\u6570\u636e\u5206\u6790<\/strong><\/span><\/section>\n
        1. \u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff08Differential Expression Analysis\uff09\uff1a\u901a\u8fc7\u7edf\u8ba1\u65b9\u6cd5\u8bc6\u522b\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002<\/span><\/section>\n
        2. \u86cb\u767d-\u86cb\u767d\u4e92\u4f5c\u7f51\u7edc\u5206\u6790\uff08Protein-Protein Interaction Network Analysis\uff09\uff1a\u4f7f\u7528STRING, Cytoscape\u7b49\u5de5\u5177\u6784\u5efa\u86cb\u767d-\u86cb\u767d\u4e92\u4f5c\u7f51\u7edc\uff0c\u8bc6\u522b\u5173\u952e\u8282\u70b9\u548c\u6a21\u5757\u3002<\/span><\/section>\n

        \u4f8b\u5982\uff1a\u7528DNAJC5\u67e5\u8be2STRING\u86cb\u767d-\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7f51\u7edc\u3002\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u5f69\u8272\u7ebf\u8868\u793a\u5404\u79cd\u7c7b\u578b\u7684\u76f8\u4e92\u4f5c\u7528\u8bc1\u636e\u3002<\/span><\/p>\n


        <\/span><\/p>\n

        <\/p>\n


        <\/span><\/p>\n

        3. \u86cb\u767d\u8d28\u529f\u80fd\u5bcc\u96c6\u5206\u6790\uff08Protein Function Enrichment Analysis\uff09\uff1a\u7c7b\u4f3c\u4e8e\u8f6c\u5f55\u7ec4\u7684\u529f\u80fd\u5bcc\u96c6\u5206\u6790\uff0c\u5bf9\u5dee\u5f02\u8868\u8fbe\u86cb\u767d\u8d28\u8fdb\u884cGO\u3001KEGG\u5bcc\u96c6\u5206\u6790\u3002<\/span><\/section>\n

        4. \u7ffb\u8bd1\u540e\u4fee\u9970\u5206\u6790\uff08Post-translational Modification Analysis\uff09\uff1a\u8bc6\u522b\u5e76\u5206\u6790\u86cb\u767d\u8d28\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\uff08\u5982\u78f7\u9178\u5316\u3001\u4e59\u9170\u5316\u3001\u6cdb\u7d20\u5316\uff09\uff0c\u7814\u7a76\u5176\u529f\u80fd\u610f\u4e49\u3002<\/span><\/p>\n


        <\/p>\n


        <\/p>\n

        \n
        \n
        \n
        \n
        \n

        <\/section>\n

        <\/section>\n
        \n
        <\/svg><\/section>\n<\/section>\n<\/section>\n
        \n

        \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66<\/strong><\/span><\/strong><\/strong><\/p>\n<\/section>\n

        \n

        <\/section>\n

        <\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/h3>\n


        <\/p>\n

        \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66<\/span><\/strong>\uff08Single-cell Transcriptomics\uff09\u662f\u7814\u7a76\u5355\u4e2a\u7ec6\u80de\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u6280\u672f\uff0c\u65e8\u5728\u63ed\u793a\u7ec6\u80de\u95f4\u7684\u5f02\u8d28\u6027\u548c\u4e2a\u4f53\u7ec6\u80de\u5728\u4e0d\u540c\u751f\u7269\u8fc7\u7a0b\u4e2d\u7684\u7279\u5b9a\u89d2\u8272\u3002\u901a\u8fc7\u5bf9\u5355\u7ec6\u80de\u7684RNA\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u80fd\u591f\u63d0\u4f9b\u9ad8\u5206\u8fa8\u7387\u7684\u57fa\u56e0\u8868\u8fbe\u8c31\uff0c\u63ed\u793a\u7ec4\u7ec7\u548c\u5668\u5b98\u5728\u5206\u5b50\u6c34\u5e73\u4e0a\u7684\u590d\u6742\u6027\u3002\u4ee5\u4e0b\u662f\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u8be6\u7ec6\u4ecb\u7ecd\uff1a<\/span><\/p>\n


        <\/p>\n

        \u4e00\u3001\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u57fa\u672c\u6982\u5ff5\u4e0e\u6570\u636e\u63cf\u8ff0<\/strong><\/span><\/p>\n


        <\/span><\/p>\n

        \u6307\u5728\u5355\u4e2a\u7ec6\u80de\u6c34\u5e73\u4e0a\uff0c\u67d0\u4e00\u7279\u5b9a\u65f6\u95f4\u70b9\u548c\u6761\u4ef6\u4e0b\u7684\u6240\u6709RNA\u8f6c\u5f55\u4ea7\u7269\u7684\u96c6\u5408\u3002\u901a\u8fc7\u5206\u6790\u5355\u4e2a\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u8868\u8fbe\uff0c\u7814\u7a76\u7ec6\u80de\u5f02\u8d28\u6027\u3001\u7ec6\u80de\u547d\u8fd0\u51b3\u5b9a\u53ca\u5176\u5728\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u7684\u529f\u80fd\u3002<\/span><\/p>\n


        <\/p>\n

        \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u901a\u5e38\u4e5f\u88ab\u7ec4\u7ec7\u6210\u4e00\u4e2a\u4e8c\u7ef4\u77e9\u9635\uff0c\u6bcf\u4e00\u884c\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u6bcf\u4e00\u5217\u4ee3\u8868\u4e00\u4e2a\u5355\u7ec6\u80de\uff0c\u8868\u793a\u6bcf\u4e2a\u57fa\u56e0\u5728\u4e0d\u540c\u7ec6\u80de\u4e2d\u7684\u8868\u8fbe\u6c34\u5e73\uff0c\u53ef\u4ee5\u53cd\u6620\u5176\u5728\u5355\u7ec6\u80de\u5c42\u6b21\u4e0a\u7684\u6d3b\u8dc3\u7a0b\u5ea6\u548c\u529f\u80fd\u72b6\u6001\u3002<\/span><\/p>\n


        <\/p>\n

        \u4e8c\u3001\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u7814\u7a76\u65b9\u6cd5<\/strong><\/span><\/section>\n
        \u5355\u7ec6\u80de\u5206\u79bb\uff1a<\/strong><\/span><\/section>\n
          \n
        1. \n
          \u8367\u5149\u6fc0\u6d3b\u7ec6\u80de\u5206\u9009\uff08FACS\uff09\uff1a\u5229\u7528\u8367\u5149\u6807\u8bb0\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u9009\u5355\u4e2a\u7ec6\u80de\u3002<\/span><\/section>\n<\/li>\n
        2. \n
          \u5fae\u6d41\u63a7\u82af\u7247\u6280\u672f\uff1a\u901a\u8fc7\u5fae\u6d41\u63a7\u88c5\u7f6e\u6355\u83b7\u548c\u5206\u79bb\u5355\u4e2a\u7ec6\u80de\u3002<\/span><\/section>\n<\/li>\n
        3. \n
          \u6fc0\u5149\u6355\u83b7\u663e\u5fae\u5207\u5272\uff08LCM\uff09\uff1a\u5229\u7528\u6fc0\u5149\u663e\u5fae\u5207\u5272\u6280\u672f\u4ece\u7ec4\u7ec7\u5207\u7247\u4e2d\u5206\u79bb\u5355\u4e2a\u7ec6\u80de\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


          <\/p>\n

          \u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff08scRNA-Seq\uff09\uff1a<\/strong><\/span><\/section>\n
            \n
          1. \n
            Smart-seq\uff1a\u6269\u589e\u5355\u7ec6\u80deRNA\uff0c\u9002\u7528\u4e8e\u5168\u957f\u8f6c\u5f55\u672c\u6d4b\u5e8f\u3002<\/span><\/section>\n<\/li>\n
          2. \n
            Drop-seq\uff1a\u901a\u8fc7\u5fae\u6ef4\u6280\u672f\u5c06\u5355\u4e2a\u7ec6\u80de\u548c\u5fae\u73e0\u5305\u88f9\u5728\u4e00\u8d77\uff0c\u8fdb\u884c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u3002<\/span><\/section>\n<\/li>\n
          3. \n
            10x Genomics Chromium\uff1a\u91c7\u7528\u5fae\u6d41\u63a7\u5e73\u53f0\uff0c\u5b9e\u73b0\u9ad8\u901a\u91cf\u5355\u7ec6\u80de\u6d4b\u5e8f\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


            <\/p>\n

            \u4e09\u3001\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5b66\u7684\u5e94\u7528<\/strong><\/span><\/section>\n
              \n
            1. \n
              \u7ec6\u80de\u5f02\u8d28\u6027\u5206\u6790\uff1a\u901a\u8fc7\u89e3\u6790\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\uff0c\u63ed\u793a\u7ec4\u7ec7\u4e2d\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u7684\u5f02\u8d28\u6027\u3002<\/span><\/section>\n<\/li>\n
            2. \n
              \u7ec6\u80de\u8c31\u7cfb\u8ffd\u8e2a\uff1a\u7814\u7a76\u7ec6\u80de\u5728\u53d1\u80b2\u548c\u5206\u5316\u8fc7\u7a0b\u4e2d\u7684\u52a8\u6001\u53d8\u5316\uff0c\u8ffd\u8e2a\u7ec6\u80de\u8c31\u7cfb\u5173\u7cfb\u3002\u5728\u53d1\u80b2\u751f\u7269\u5b66\u3001\u518d\u751f\u533b\u5b66\u7b49\u9886\u57df\u5177\u6709\u91cd\u8981\u5e94\u7528\u3002<\/span><\/section>\n<\/li>\n
            3. \n
              \u75be\u75c5\u7814\u7a76\uff1a\u901a\u8fc7\u5355\u7ec6\u80de\u5206\u6790\uff0c\u63ed\u793a\u75be\u75c5\u7ec4\u7ec7\u4e2d\u7684\u7ec6\u80de\u5f02\u8d28\u6027\u548c\u7279\u5f02\u6027\u7ec6\u80de\u7c7b\u578b\u3002\u5e2e\u52a9\u7406\u89e3\u75be\u75c5\u7684\u53d1\u751f\u548c\u53d1\u5c55\u673a\u5236\uff0c\u5982\u764c\u75c7\u4e2d\u7684\u80bf\u7624\u5fae\u73af\u5883\u3001\u514d\u75ab\u9003\u9038\u673a\u5236\u7b49\u3002<\/span><\/section>\n<\/li>\n
            4. \n
              \u836f\u7269\u53cd\u5e94\u548c\u8010\u836f\u673a\u5236\u7814\u7a76\uff1a\u7814\u7a76\u5355\u4e2a\u7ec6\u80de\u5bf9\u836f\u7269\u7684\u53cd\u5e94\uff0c\u63ed\u793a\u8010\u836f\u7ec6\u80de\u7684\u7279\u5f81\u548c\u673a\u5236\u3002\u4e3a\u4e2a\u6027\u5316\u6cbb\u7597\u548c\u65b0\u836f\u5f00\u53d1\u63d0\u4f9b\u4f9d\u636e\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


              <\/p>\n

              \u56db\u3001\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u5206\u6790<\/strong><\/span><\/section>\n
              1. \u7ec6\u80de\u805a\u7c7b\u5206\u6790\uff08Cell Clustering Analysis\uff09\uff1a\u964d\u7ef4\uff08\u5982PCA, t-SNE, UMAP\uff09\u548c\u7ec6\u80de\u805a\u7c7b\uff08\u5982Louvain, K-means\uff09\uff0c\u7528\u4e8e\u8bc6\u522b\u4e0d\u540c\u7684\u7ec6\u80de\u7fa4\u4f53\u3002<\/span><\/section>\n

              \u4f8b\u5982\uff1a\u901a\u8fc7PCA\u3001tSNE\u548cUMAP\u65b9\u5f0f\u5bf9\u7ec6\u80de\u8fdb\u884c\u964d\u7ef4\u5206\u6790<\/span><\/p>\n


              <\/span><\/p>\n

              <\/p>\n


              <\/span><\/p>\n

              \u7ec6\u80de\u7c7b\u578b\u6ce8\u91ca\uff08Cell Type Annotation\uff09\uff1a\u57fa\u4e8e\u5df2\u77e5\u7684\u6807\u5fd7\u57fa\u56e0\u6216\u4f7f\u7528\u81ea\u52a8\u5316\u6ce8\u91ca\u5de5\u5177\uff08\u5982SingleR, Garnett\uff09\u5bf9\u805a\u7c7b\u7ed3\u679c\u8fdb\u884c\u7ec6\u80de\u7c7b\u578b\u6807\u6ce8\u3002
              \u4f8b\u5982\uff1a\u901a\u8fc7\u7ecf\u5178\u7684\u7ec6\u80de\u5206\u5b50\u6807\u5fd7\u7269\u6765\u6ce8\u91ca\u7ed3\u76f4\u80a0\u764c\u7684\u7ec6\u80de\u4e9a\u7fa4<\/span><\/section>\n

              <\/span><\/section>\n

              <\/p>\n


              <\/span><\/p>\n

              2. \u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff08Differential Expression Analysis\uff09\uff1a\u6bd4\u8f83\u4e0d\u540c\u7ec6\u80de\u7fa4\u4f53\u6216\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\uff0c\u4f7f\u7528\u5de5\u5177\u5982DESeq2, edgeR, MAST\u8fdb\u884c\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u9274\u5b9a\u3002<\/span><\/section>\n
              3. \u4e9a\u7fa4\u7279\u5f02\u6027\u57fa\u56e0\u8868\u8fbe\u5206\u6790\uff08Subpopulation-specific Gene Expression Analysis\uff09\uff1a\u8bc6\u522b\u548c\u5206\u6790\u7279\u5b9a\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u7279\u5f02\u6027\u9ad8\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u7814\u7a76\u5176\u751f\u7269\u5b66\u529f\u80fd\u3002<\/span><\/section>\n
              4. \u4f2a\u65f6\u5e8f\u5206\u6790\uff08Pseudotime Analysis\uff09\uff1a\u4f7f\u7528Monocle, Slingshot, scVelo\u7b49\u5de5\u5177\u63a8\u65ad\u7ec6\u80de\u53d1\u80b2\u8f68\u8ff9\u6216\u52a8\u6001\u53d8\u5316\uff0c\u63a2\u7d22\u7ec6\u80de\u5206\u5316\u8def\u5f84\u3002<\/span><\/section>\n

              \u4f8b\u5982\uff1a\u901a\u8fc74\u79cd\u4f2a\u65f6\u5e8f\u5206\u6790\u7b97\u6cd5\u5b9a\u4f4d\u5230\u4e2d\u6027\u7c92\u7ec6\u80de\u7684\u7ec8\u672b\u5206\u5316\u4e9a\u7fa4<\/span><\/p>\n


              <\/span><\/p>\n

              <\/p>\n


              <\/span><\/p>\n

              5. \u7ec6\u80de\u95f4\u901a\u8baf\u5206\u6790\uff08Cell-Cell Communication Analysis\uff09\uff1a\u4f7f\u7528CellPhoneDB, NicheNet, CellChat\u7b49\u5de5\u5177\u5206\u6790\u7ec6\u80de\u95f4\u7684\u914d\u4f53-\u53d7\u4f53\u76f8\u4e92\u4f5c\u7528\uff0c\u7814\u7a76\u7ec6\u80de\u95f4\u901a\u8baf\u673a\u5236\u3002<\/span><\/section>\n

              \u4f8b\u5982\uff1a\u57fa\u4e8eCellChat\u7814\u7a76\u80bf\u7624\u7ec6\u80de\u548c\u5176\u5b83\u7ec6\u80de\u7684\u7ec6\u80de\u901a\u8baf\u5f3a\u5ea6<\/span><\/p>\n


              <\/span><\/p>\n

              <\/p>\n


              <\/span><\/p>\n

              6. \u8f6c\u5f55\u56e0\u5b50\u8c03\u63a7\u7f51\u7edc\u5206\u6790\uff08Transcription Factor Regulatory Network Analysis\uff09\uff1a\u4f7f\u7528SCENIC\u7b49\u5de5\u5177\u8bc6\u522b\u5173\u952e\u8f6c\u5f55\u56e0\u5b50\u53ca\u5176\u8c03\u63a7\u7684\u57fa\u56e0\u7f51\u7edc\uff0c\u7814\u7a76\u8f6c\u5f55\u8c03\u63a7\u673a\u5236\u3002<\/span><\/section>\n

              \u4f8b\u5982\uff1a\u57fa\u4e8eSCENIC\u6765\u5206\u6790\u8010\u836f\u7ec6\u80de\u682a\u7684\u9a71\u52a8\u8f6c\u5f55\u56e0\u5b50<\/span><\/p>\n


              <\/span><\/p>\n

              <\/p>\n


              <\/span><\/p>\n

              7. \u7ec6\u80de\u5468\u671f\u72b6\u6001\u5206\u6790\uff08Cell Cycle State Analysis\uff09\uff1a\u4f7f\u7528Cyclone, Seurat\u7b49\u5de5\u5177\u8bc4\u4f30\u5355\u7ec6\u80de\u7684\u7ec6\u80de\u5468\u671f\u72b6\u6001\uff0c\u7814\u7a76\u7ec6\u80de\u589e\u6b96\u548c\u5206\u88c2\u7684\u52a8\u6001\u53d8\u5316\u3002<\/span><\/section>\n
              8. \u57fa\u56e0\u8868\u8fbe\u53d8\u5316\u7684\u8f68\u8ff9\u5206\u6790\uff08Trajectory Analysis of Gene Expression Changes\uff09\uff1a\u4f7f\u7528RNA velocity\u7b49\u65b9\u6cd5\u63a8\u65ad\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\u7684\u65b9\u5411\u548c\u901f\u7387\uff0c\u5206\u6790\u7ec6\u80de\u72b6\u6001\u7684\u52a8\u6001\u8f6c\u53d8\u3002<\/span><\/section>\n
              9. \u5355\u7ec6\u80de\u4e9a\u7fa4\u5f02\u8d28\u6027\u5206\u6790\uff08Heterogeneity Analysis of Single-cell Subpopulations\uff09\uff1a\u6df1\u5165\u5206\u6790\u4e0d\u540c\u7ec6\u80de\u4e9a\u7fa4\u7684\u5f02\u8d28\u6027\uff0c\u7814\u7a76\u5176\u5728\u4e0d\u540c\u751f\u7269\u5b66\u6761\u4ef6\u4e0b\u7684\u53d8\u5316\u3002<\/span><\/section>\n

              10. \u7ec6\u80de\u8c31\u7cfb\u6811\u6784\u5efa\uff08Cell Lineage Tree Construction\uff09\uff1a\u4f7f\u7528\u5de5\u5177\u5982TSCAN, FateID\u6784\u5efa\u7ec6\u80de\u8c31\u7cfb\u6811\uff0c\u7814\u7a76\u7ec6\u80de\u5206\u5316\u8def\u5f84\u548c\u8c31\u7cfb\u5173\u7cfb\u3002<\/span><\/p>\n


              <\/p>\n


              <\/p>\n

              \n
              \n
              \n
              \n
              \n

              <\/section>\n

              <\/section>\n
              \n
              <\/svg><\/section>\n<\/section>\n<\/section>\n
              \n

              \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66<\/strong><\/span><\/strong><\/strong><\/p>\n<\/section>\n

              \n

              <\/section>\n

              <\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/h3>\n


              <\/p>\n

              \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\uff08Spatial Transcriptomics\uff09\u662f\u4e00\u79cd\u7ed3\u5408\u7a7a\u95f4\u4fe1\u606f\u548c\u8f6c\u5f55\u7ec4\u5b66\u6570\u636e\u7684\u6280\u672f\uff0c\u65e8\u5728\u63ed\u793a\u7ec4\u7ec7\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u7a7a\u95f4\u5206\u5e03\u3002\u901a\u8fc7\u5728\u7ec4\u7ec7\u5207\u7247\u4e0a\u4fdd\u6301\u7ec6\u80de\u7a7a\u95f4\u4f4d\u7f6e\u7684\u540c\u65f6\uff0c\u8fdb\u884cRNA\u6d4b\u5e8f\uff0c\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u53ef\u4ee5\u63d0\u4f9b\u7ec4\u7ec7\u7ed3\u6784\u548c\u529f\u80fd\u7684\u9ad8\u5206\u8fa8\u7387\u56fe\u8c31\u3002\u4ee5\u4e0b\u662f\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7684\u8be6\u7ec6\u4ecb\u7ecd\uff1a<\/span><\/p>\n


              <\/p>\n

              \u4e00\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7684\u57fa\u672c\u6982\u5ff5\u4e0e\u6570\u636e\u63cf\u8ff0<\/strong><\/span><\/p>\n


              <\/strong><\/span><\/p>\n

              \u7a7a\u95f4\u8f6c\u5f55\u7ec4\uff1a\u6307\u5728\u4fdd\u7559\u7ec6\u80de\u7a7a\u95f4\u4f4d\u7f6e\u4fe1\u606f\u7684\u60c5\u51b5\u4e0b\uff0c\u5bf9\u7279\u5b9a\u7ec4\u7ec7\u6216\u5668\u5b98\u4e2d\u6240\u6709RNA\u8f6c\u5f55\u4ea7\u7269\u7684\u96c6\u5408\u3002\u901a\u8fc7\u7ed3\u5408\u7a7a\u95f4\u4fe1\u606f\u548cRNA\u6d4b\u5e8f\uff0c\u7814\u7a76\u7ec4\u7ec7\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u7a7a\u95f4\u5206\u5e03\u53ca\u5176\u5728\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u3002<\/span><\/p>\n


              <\/p>\n

              \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u7ecf\u5e38\u88ab\u7ec4\u7ec7\u6210\u4e00\u4e2a\u4e8c\u7ef4\u77e9\u9635\uff0c\u6bcf\u4e00\u884c\u4ee3\u8868\u4e00\u4e2a\u57fa\u56e0\uff0c\u6bcf\u4e00\u5217\u4ee3\u8868\u4e00\u4e2a\u7a7a\u95f4\u4f4d\u7f6e\u6216\u533a\u57df\uff0c\u8868\u793a\u6bcf\u4e2a\u57fa\u56e0\u5728\u4e0d\u540c\u7a7a\u95f4\u4f4d\u7f6e\u7684\u8868\u8fbe\u6c34\u5e73\u53ef\u4ee5\u53cd\u6620\u5176\u5728\u7279\u5b9a\u7ec4\u7ec7\u7ed3\u6784\u4e2d\u7684\u6d3b\u8dc3\u7a0b\u5ea6\u548c\u529f\u80fd\u72b6\u6001\u3002\u8fd9\u4e9b\u4f4d\u7f6e\u53ef\u4ee5\u662f\u7ec4\u7ec7\u5207\u7247\u4e0a\u7684\u4e0d\u540c\u70b9\uff0c\u901a\u8fc7\u6280\u672f\u5982\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u6d4b\u5e8f\u6216\u5176\u4ed6\u7a7a\u95f4\u5206\u8fa8\u7684\u6d4b\u5e8f\u65b9\u6cd5\u83b7\u5f97\u3002\u4f4d\u7f6e\u4fe1\u606f\u901a\u5e38\u4ee5\u5750\u6807\u5f62\u5f0f\uff08\u5982 x \u548c y \u5750\u6807\uff09\u5b58\u50a8\uff0c\u8868\u793a\u5728\u7ec4\u7ec7\u5207\u7247\u4e0a\u7684\u5177\u4f53\u4f4d\u7f6e\u3002<\/span><\/p>\n


              <\/p>\n

              \u4e8c\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7684\u7814\u7a76\u65b9\u6cd5<\/strong><\/span><\/section>\n
              10x Genomics Visium\uff1aVisium Spatial Gene Expression \u901a\u8fc7\u5728\u73bb\u7247\u4e0a\u8fdb\u884c\u7a7a\u95f4\u6761\u5f62\u7801\u6807\u8bb0\uff0c\u5c06\u7ec4\u7ec7\u5207\u7247\u56fa\u5b9a\u5728\u73bb\u7247\u4e0a\uff0c\u7136\u540e\u5bf9\u6bcf\u4e2a\u4f4d\u7f6e\u7684RNA\u8fdb\u884c\u6355\u83b7\u3001\u9006\u8f6c\u5f55\u548c\u6d4b\u5e8f\u3002\u6bcf\u4e2a\u6761\u5f62\u7801\u5bf9\u5e94\u4e00\u4e2a\u7279\u5b9a\u7684\u7a7a\u95f4\u4f4d\u7f6e\u3002<\/span><\/section>\n

              \u4f18\u70b9\uff1a\u5177\u6709\u8f83\u9ad8\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\uff08\u7ea655\u5fae\u7c73\uff09\uff0c\u80fd\u591f\u751f\u6210\u7ec4\u7ec7\u7684\u9ad8\u8d28\u91cf\u7a7a\u95f4\u8868\u8fbe\u56fe\u8c31\uff0c\u9002\u7528\u4e8e\u5404\u79cd\u7ec4\u7ec7\u7c7b\u578b\u7684\u7814\u7a76\u3002<\/span><\/p>\n


              <\/p>\n

              HDST\uff08High Definition Spatial Transcriptomics\uff09\uff1aHDST\u4f7f\u7528\u8d85\u9ad8\u5bc6\u5ea6\u7684\u7a7a\u95f4\u6761\u5f62\u7801\u9635\u5217\uff0c\u6bcf\u4e2a\u6761\u5f62\u7801\u70b9\u7684\u5927\u5c0f\u4e3a2\u5fae\u7c73\uff0c\u5b9e\u73b0\u66f4\u9ad8\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\u3002\u901a\u8fc7\u6355\u83b7\u6bcf\u4e2a\u4f4d\u7f6e\u7684RNA\u5e76\u8fdb\u884c\u6d4b\u5e8f\uff0c\u751f\u6210\u9ad8\u5206\u8fa8\u7387\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u56fe\u8c31\u3002<\/span><\/section>\n

              \u4f18\u70b9\uff1a\u6781\u9ad8\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\uff082\u5fae\u7c73\uff09\uff0c\u80fd\u591f\u89e3\u6790\u7ec6\u80de\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\u5f02\u8d28\u6027\uff0c\u9002\u7528\u4e8e\u7814\u7a76\u590d\u6742\u7ec4\u7ec7\u7684\u5fae\u89c2\u7ed3\u6784\u3002<\/span><\/p>\n


              <\/p>\n

              Stereo-seq\uff08Spatial Enhanced Resolution Omics-sequencing\uff09\uff1aStereo-seq\u901a\u8fc7\u5728\u73bb\u7247\u4e0a\u94fa\u8bbe\u5305\u542b\u7279\u5b9a\u6761\u5f62\u7801\u7684\u7eb3\u7c73\u73e0\u9635\u5217\uff0c\u7136\u540e\u5c06\u7ec4\u7ec7\u5207\u7247\u653e\u7f6e\u5728\u4e0a\u9762\uff0c\u8fdb\u884cRNA\u6355\u83b7\u548c\u6d4b\u5e8f\u3002\u6bcf\u4e2a\u7eb3\u7c73\u73e0\u643a\u5e26\u552f\u4e00\u7684\u6761\u5f62\u7801\uff0c\u63d0\u4f9b\u8d85\u9ad8\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\u3002<\/span><\/section>\n

              \u4f18\u70b9\uff1a\u7eb3\u7c73\u7ea7\u7a7a\u95f4\u5206\u8fa8\u7387\uff08\u7ea6500\u7eb3\u7c73\uff09\uff0c\u80fd\u591f\u8be6\u7ec6\u89e3\u6790\u7ec6\u80de\u5185\u7ed3\u6784\u548c\u57fa\u56e0\u8868\u8fbe\uff0c\u9002\u7528\u4e8e\u9ad8\u7cbe\u5ea6\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5206\u6790\u3002<\/span><\/p>\n


              <\/p>\n

              \u4e09\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7684\u5e94\u7528<\/strong><\/span><\/span><\/section>\n
                \n
              1. \n
                \u7ec4\u7ec7\u7ed3\u6784\u4e0e\u529f\u80fd\u5206\u6790\uff1a\u901a\u8fc7\u89e3\u6790\u7ec4\u7ec7\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u7a7a\u95f4\u5206\u5e03\uff0c\u63ed\u793a\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u548c\u533a\u57df\u7684\u529f\u80fd\u5dee\u5f02\u3002\u6bd4\u5982\uff1a\u7814\u7a76\u764c\u75c7\u7ec4\u7ec7\u4e2d\u80bf\u7624\u7ec6\u80de\u3001\u514d\u75ab\u7ec6\u80de\u548c\u57fa\u8d28\u7ec6\u80de\u7684\u7a7a\u95f4\u5206\u5e03\u53ca\u5176\u76f8\u4e92\u4f5c\u7528\uff0c\u5e2e\u52a9\u7406\u89e3\u80bf\u7624\u5fae\u73af\u5883\u7684\u590d\u6742\u6027\u548c\u5f02\u8d28\u6027\u3002<\/span><\/section>\n<\/li>\n
              2. \n
                \u5fae\u73af\u5883\u7814\u7a76\uff1a\u7814\u7a76\u7ec4\u7ec7\u4e2d\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u53ca\u5176\u5fae\u73af\u5883\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u63ed\u793a\u7ec6\u80de\u95f4\u7684\u901a\u4fe1\u548c\u8c03\u63a7\u673a\u5236\u3002\u6bd4\u5982\uff1a\u5728\u795e\u7ecf\u79d1\u5b66\u4e2d\uff0c\u7814\u7a76\u5927\u8111\u4e2d\u795e\u7ecf\u5143\u548c\u80f6\u8d28\u7ec6\u80de\u7684\u7a7a\u95f4\u5206\u5e03\u548c\u529f\u80fd\u8fde\u63a5\uff0c\u7406\u89e3\u795e\u7ecf\u7f51\u7edc\u7684\u7ed3\u6784\u548c\u529f\u80fd\u3002<\/span><\/section>\n<\/li>\n
              3. \n
                \u75be\u75c5\u673a\u5236\u7814\u7a76\uff1a\u901a\u8fc7\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\uff0c\u63ed\u793a\u75be\u75c5\u7ec4\u7ec7\u4e2d\u7684\u7279\u5b9a\u7ec6\u80de\u7c7b\u578b\u548c\u533a\u57df\u7684\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\u3002\u6bd4\u5982\uff1a\u5728\u5fc3\u8840\u7ba1\u75be\u75c5\u7814\u7a76\u4e2d\uff0c\u5206\u6790\u5fc3\u808c\u6897\u6b7b\u533a\u57df\u5185\u4e0d\u540c\u7ec6\u80de\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\uff0c\u63ed\u793a\u5fc3\u808c\u4fee\u590d\u548c\u91cd\u5851\u8fc7\u7a0b\u4e2d\u7684\u5173\u952e\u57fa\u56e0\u548c\u8def\u5f84\u3002<\/span><\/section>\n<\/li>\n
              4. \n
                \u53d1\u80b2\u4e0e\u518d\u751f\u7814\u7a76\uff1a\u7814\u7a76\u80da\u80ce\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u7a7a\u95f4\u52a8\u6001\u53d8\u5316\uff0c\u63ed\u793a\u53d1\u80b2\u8c03\u63a7\u673a\u5236\u3002\u6bd4\u5982\uff1a\u5728\u53d1\u80b2\u751f\u7269\u5b66\u4e2d\uff0c\u7814\u7a76\u80da\u80ce\u4e0d\u540c\u53d1\u80b2\u9636\u6bb5\u7684\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\uff0c\u7406\u89e3\u5668\u5b98\u53d1\u80b2\u548c\u7ec6\u80de\u5206\u5316\u7684\u7a7a\u95f4\u8c03\u63a7\u3002<\/span><\/section>\n<\/li>\n
              5. \n

                \u836f\u7269\u53cd\u5e94\u548c\u8010\u836f\u673a\u5236\u7814\u7a76\uff1a\u5206\u6790\u836f\u7269\u5904\u7406\u524d\u540e\u7ec4\u7ec7\u4e2d\u57fa\u56e0\u8868\u8fbe\u7684\u7a7a\u95f4\u53d8\u5316\uff0c\u63ed\u793a\u8010\u836f\u7ec6\u80de\u7684\u7279\u5f81\u548c\u673a\u5236\u3002\u6bd4\u5982\uff1a\u5728\u80bf\u7624\u5b66\u4e2d\uff0c\u7814\u7a76\u6297\u764c\u836f\u7269\u5904\u7406\u540e\u7684\u80bf\u7624\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u53d8\u5316\uff0c\u5bfb\u627e\u8010\u836f\u7ec6\u80de\u53ca\u5176\u7a7a\u95f4\u5206\u5e03\uff0c\u4e3a\u4e2a\u6027\u5316\u6cbb\u7597\u63d0\u4f9b\u4f9d\u636e\u3002<\/span><\/p>\n<\/li>\n

              6. \n

                \u751f\u7269\u6807\u5fd7\u7269\u53d1\u73b0\uff1a\u901a\u8fc7\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\uff0c\u5bfb\u627e\u75be\u75c5\u7279\u5f02\u6027\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u548c\u7a7a\u95f4\u5206\u5e03\u7279\u5f81\uff0c\u4f5c\u4e3a\u6f5c\u5728\u7684\u751f\u7269\u6807\u5fd7\u7269\u3002\u6bd4\u5982\uff1a\u5728\u809d\u75c5\u7814\u7a76\u4e2d\uff0c\u53d1\u73b0\u809d\u7ea4\u7ef4\u5316\u533a\u57df\u5185\u7279\u5f02\u6027\u8868\u8fbe\u7684\u57fa\u56e0\uff0c\u4f5c\u4e3a\u8bca\u65ad\u548c\u9884\u540e\u7684\u751f\u7269\u6807\u5fd7\u7269\u3002<\/span><\/p>\n<\/li>\n

              7. \n
                \u795e\u7ecf\u7f51\u7edc\u7814\u7a76\uff1a\u7814\u7a76\u5927\u8111\u4e2d\u795e\u7ecf\u5143\u548c\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\u7684\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\uff0c\u63ed\u793a\u795e\u7ecf\u7f51\u7edc\u7684\u529f\u80fd\u8fde\u63a5\u548c\u8c03\u63a7\u673a\u5236\u3002\u6bd4\u5982\uff1a\u5728\u963f\u5c14\u8328\u6d77\u9ed8\u75c5\u7814\u7a76\u4e2d\uff0c\u5206\u6790\u5927\u8111\u4e0d\u540c\u533a\u57df\u7684\u57fa\u56e0\u8868\u8fbe\uff0c\u7406\u89e3\u75be\u75c5\u8fc7\u7a0b\u4e2d\u795e\u7ecf\u5143\u548c\u80f6\u8d28\u7ec6\u80de\u7684\u53d8\u5316\u548c\u76f8\u4e92\u4f5c\u7528\u3002<\/span><\/section>\n<\/li>\n<\/ol>\n


                <\/p>\n

                \u56db\u3001\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u5206\u6790<\/strong><\/span><\/section>\n

                1. \u4f7f\u7528\u964d\u7ef4\u65b9\u6cd5\uff08\u5982PCA, t-SNE, UMAP\uff09\u548c\u805a\u7c7b\u7b97\u6cd5\uff08\u5982Louvain, K-means\uff09\u8fdb\u884c\u7a7a\u95f4\u70b9\u805a\u7c7b\uff0c\u8bc6\u522b\u4e0d\u540c\u7a7a\u95f4\u533a\u57df\u7684\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u3002<\/span><\/p>\n

                2. \u7a7a\u95f4\u53ef\u89c6\u5316\uff08Spatial <\/span>Visualization\uff09\uff1a<\/span>\u5c06\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u6295\u5f71\u5230\u7ec4\u7ec7\u5207\u7247\u56fe\u50cf\u4e0a\uff0c\u751f\u6210\u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u70ed\u56fe\u548c\u7a7a\u95f4\u70b9\u56fe\uff08\u5982Seurat, Scanpy\u4e2d\u7684\u7a7a\u95f4\u6a21\u5757\uff09\u3002<\/span><\/p>\n

                \u4f8b\u5982\uff1a\u5728HD\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u4e2d\u53ef\u89c6\u5316FCGBP\u7684\u8868\u8fbe\uff0c\u8be5\u5206\u5b50\u6807\u5fd7\u7269\u662f\u676f\u72b6\u7ec6\u80de\u7684marker<\/span><\/p>\n

                <\/p>\n

                3. \u5dee\u5f02\u8868\u8fbe\u5206\u6790\uff08Differential Expression Analysis\uff09\uff1a\u6bd4\u8f83\u4e0d\u540c\u7a7a\u95f4\u533a\u57df\u6216\u6761\u4ef6\u4e0b\u7684\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\uff0c\u4f7f\u7528DESeq2, edgeR\u7b49\u5de5\u5177\u8bc6\u522b\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u3002<\/span><\/section>\n
                4. \u7a7a\u95f4\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u5206\u6790\uff08Spatial Gene Expression Pattern Analysis\uff09\uff1a<\/span>\u8bc6\u522b\u548c\u5206\u6790\u7279\u5b9a\u7a7a\u95f4\u533a\u57df\u7684\u7279\u5f02\u6027\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\uff0c\u4f7f\u7528SpatialDE, SPARK\u7b49\u5de5\u5177\u3002<\/span><\/section>\n
                5. \u7a7a\u95f4\u57df\u9274\u5b9a\uff08Spatial Domain Identification\uff09\uff1a<\/span>\u4f7f\u7528BayesSpace, stLearn\u7b49\u5de5\u5177\u8bc6\u522b\u548c\u6ce8\u91ca\u4e0d\u540c\u7684\u7a7a\u95f4\u57df\uff0c\u7814\u7a76\u7ec4\u7ec7\u7ed3\u6784\u548c\u529f\u80fd\u3002<\/span><\/section>\n
                6. \u7a7a\u95f4\u53ef\u89c6\u5316\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\uff08Spatial Co-expression Network Visualization\uff09\uff1a<\/span>\u6784\u5efa\u548c\u53ef\u89c6\u5316\u7a7a\u95f4\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\uff0c\u8bc6\u522b\u7a7a\u95f4\u4e0a\u5171\u8868\u8fbe\u7684\u57fa\u56e0\u6a21\u5757\u3002<\/span><\/section>\n
                7. \u7a7a\u95f4\u5dee\u5f02\u57fa\u56e0\u5171\u8868\u8fbe\u5206\u6790\uff08Spatial Differential Gene Co-expression Analysis\uff09\uff1a<\/span>\u6bd4\u8f83\u4e0d\u540c\u7a7a\u95f4\u533a\u57df\u7684\u57fa\u56e0\u5171\u8868\u8fbe\u6a21\u5f0f\uff0c\u5206\u6790\u7a7a\u95f4\u5f02\u8d28\u6027\u3002<\/span><\/section>\n
                8. \u7a7a\u95f4\u5206\u89e3\u5206\u6790\uff08Spatial Deconvolution Analysis\uff09\uff1a<\/span>\u4f7f\u7528\u5de5\u5177\u5982SPOTlight, Cell2location\u8fdb\u884c\u7ec6\u80de\u7c7b\u578b\u5206<\/span>\u89e3\uff0c\u5c06\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6570\u636e\u4e0e\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u6574\u5408\uff0c\u63a8\u65ad\u6bcf\u4e2a\u7a7a\u95f4\u70b9\u7684\u7ec6\u80de\u7c7b\u578b\u7ec4\u6210\u3002<\/span><\/section>\n

                \u4f8b\u5982\uff1a\u57fa\u4e8eCell2Location\u53cd\u5377\u79ef\u7a7a\u95f4Spot\u7684\u7ec6\u80de\u7ec4\u6210<\/span><\/p>\n


                <\/span><\/p>\n

                <\/p>\n


                <\/span><\/p>\n

                9. \u7a7a\u95f4\u901a\u8def\u5206\u6790\uff08Spatial Pathway Analysis\uff09\uff1a\u8fdb\u884c\u901a\u8def\u5bcc\u96c6\u5206\u6790\uff0c\u7814\u7a76\u7279\u5b9a\u7a7a\u95f4\u533a\u57df\u7684\u901a\u8def\u6d3b\u6027\u548c\u529f\u80fd\u3002<\/span><\/section>\n

                \u4f8b\u5982\uff1a\u67e5\u770b\u7a7a\u8f6c\u4e2d\u6bcf\u4e2aSpot\u7684\u901a\u8def\u6d3b\u6027\u8bc4\u5206\uff0c\u4ee5\u6b64\u6765\u5206\u6790\u7a7a\u95f4\u4e2d\u7684\u901a\u8def\u6fc0\u6d3b\u72b6\u6001\u3002<\/span><\/p>\n


                <\/span><\/p>\n

                <\/p>\n


                <\/span><\/p>\n

                10. \u7a7a\u95f4\u57df\u5dee\u5f02\u5206\u6790\uff08Spatial Domain Differential Analysis\uff09\uff1a\u6bd4\u8f83\u4e0d\u540c\u7a7a\u95f4\u57df\u7684\u57fa\u56e0\u8868\u8fbe\u548c\u529f\u80fd\u7279\u5f81\uff0c\u7814\u7a76\u7a7a\u95f4\u57df\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/p>\n


                <\/p>\n

                <\/span><\/p>\n


                <\/span><\/section>\n
                11. \u7a7a\u95f4\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\u6784\u5efa\uff08Spatial Gene Co-expression Network Construction\uff09\uff1a\u4f7f\u7528SpaceFlow\u7b49\u5de5\u5177\u6784\u5efa\u7a7a\u95f4\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\uff0c\u7814\u7a76\u57fa\u56e0\u5728\u7a7a\u95f4\u4e0a\u7684\u5171\u8868\u8fbe\u5173\u7cfb\u3002<\/span><\/section>\n
                12. \u7a7a\u95f4\u70b9\u6ce8\u91ca\uff08Spatial Spot Annotation\uff09\uff1a<\/span>\u57fa\u4e8e\u5df2\u77e5\u7684\u7ec6\u80de\u7c7b\u578b\u6216\u57fa\u56e0\u6807\u5fd7\u7269\uff0c\u5bf9\u7a7a\u95f4\u70b9\u8fdb\u884c\u6ce8\u91ca\uff0c\u8bc6\u522b\u4e0d\u540c\u7ec4\u7ec7\u7ed3\u6784\u4e2d\u7684\u7ec6\u80de\u7c7b\u578b\u3002<\/span><\/section>\n
                13. \u7a7a\u95f4\u591a\u7ec4\u5b66\u6574\u5408\u5206\u6790\uff08Spatial Multi-omics Integration Analysis\uff09\uff1a<\/span>\u6574\u5408\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u6570\u636e\u4e0e\u5176\u4ed6\u7ec4\u5b66\u6570\u636e\uff08\u5982\u7a7a\u95f4\u86cb\u767d\u8d28\u7ec4\u5b66\uff0c\u7a7a\u95f4\u4ee3\u8c22\u7ec4\u5b66\uff09\u8fdb\u884c\u8054\u5408\u5206\u6790\uff0c\u63ed\u793a\u66f4\u5168\u9762\u7684\u7a7a\u95f4\u751f\u7269\u5b66\u4fe1\u606f\u3002<\/span><\/section>\n
                14. \u7a7a\u95f4\u53ef\u89c6\u5316\u7ec6\u80de-\u7ec6\u80de\u901a\u8baf<\/span>\u5206\u6790\uff08Spatial Visualization of Cell-Cell Communication Analysis\uff09\uff1a<\/span>\u4f7f\u7528\u5de5\u5177\u5982NicheNet, CellPhoneDB\u8fdb\u884c\u7ec6\u80de-\u7ec6\u80de\u901a\u8baf\u5206\u6790\uff0c\u5e76\u5728\u7a7a\u95f4\u4e0a\u53ef\u89c6\u5316\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u3002<\/span><\/section>\n

                \u4f8b\u5982\uff1a\u57fa\u4e8eCellChatV2\u6765\u8ba1\u7b97\u4e0d\u540c\u4e9a\u7fa4\u5728SPP1\u4fe1\u53f7\u4e2d\u7684\u4ea4\u4e92\u5f3a\u5ea6<\/span><\/p>\n


                <\/span><\/p>\n

                <\/p>\n


                <\/p>\n


                <\/section>\n
                \n
                \n
                \n
                \n

                \u751f\u547d\u590d\u6742\u6027\u8bfb\u4e66\u4f1a\u62db\u52df\u4e2d<\/strong>
                <\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


                <\/section>\n

                \u96c6\u667a\u4ff1\u4e50\u90e8\u8054\u5408\u897f\u6e56\u5927\u5b66\u7406\u5b66\u9662\u53ca\u4ea4\u53c9\u79d1\u5b66\u4e2d\u5fc3\u8bb2\u5e2d\u6559\u6388\u6c64\u96f7\u7ff0\uff0c\u56fd\u5bb6\u86cb\u767d\u8d28\u79d1\u5b66\u4e2d\u5fc3\uff08\u5317\u4eac\uff09\u526f\u7814\u7a76\u5458\u5e38\u4e58\u3001\u674e\u6768\uff0c\u9999\u6e2f\u6d78\u4f1a\u5927\u5b66\u52a9\u7406\u6559\u6388\u5510\u4e7e\u5143\uff0c\u5317\u4eac\u5927\u5b66\u524d\u6cbf\u4ea4\u53c9\u5b66\u79d1\u7814\u7a76\u9662\u7814\u7a76\u5458\u6797\u4e00\u701a\uff0c<\/span>\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\u535a\u58eb\u540e\u5510\u8bd7\u5a55\uff0c\u5171\u540c\u53d1\u8d77\u300c\u751f\u547d\u590d\u6742\u6027\uff1a\u751f\u547d\u590d\u6742\u7cfb\u7edf\u7684\u6784\u6210\u539f\u7406<\/a>\u300d\u8bfb\u4e66\u4f1a\uff0c\u4ece\u5fae\u89c2\u7ec6\u80de\u5c3a\u5ea6\u3001\u4ecb\u89c2\u7ec4\u7ec7\u5668\u5b98\u5c3a\u5ea6\uff0c\u5230\u5b8f\u89c2\u4eba\u4f53\u5c3a\u5ea6\uff0c\u68b3\u7406\u751f\u547d\u79d1\u5b66\u9886\u57df\u4e2d\u7684\u91cd\u8981\u95ee\u9898\u4ee5\u53ca\u91cd\u8981\u6570\u636e\uff0c\u5e0c\u671b\u4fc3\u8fdb\u7edf\u8ba1\u7269\u7406\u3001\u673a\u5668\u5b66\u4e60\u65b9\u6cd5\u7814\u7a76\u8005\u548c\u7ec4\u5b66\u7814\u7a76\u8005\u4e4b\u95f4\u7684\u6df1\u5ea6\u4ea4\u6d41\uff0c\u5efa\u7acb\u8de8\u5b66\u79d1\u5408\u4f5c\u5173\u7cfb\uff0c\u6fc0\u53d1\u65b0\u7684\u7814\u7a76\u601d\u8def\u548c\u5408\u4f5c\u9879\u76ee\u3002\u8bfb\u4e66\u4f1a\u4ece2024\u5e748\u67086\u65e5\u5f00\u59cb\uff0c\u6bcf\u5468\u4e8c\u665a19:00-21:00\u8fdb\u884c\uff0c\u6301\u7eed\u65f6\u95f4\u9884\u8ba110-12\u5468\u3002\u6b22\u8fce\u5bf9\u8fd9\u4e2a\u751f\u547d\u79d1\u5b66\u3001\u7269\u7406\u5b66\u3001\u590d\u6742\u79d1\u5b66\u3001\u8ba1\u7b97\u673a\u79d1\u5b66\u6df1\u5ea6\u4ea4\u53c9\u7684\u524d\u6cbf\u9886\u57df\u611f\u5174\u8da3\u7684\u670b\u53cb\u52a0\u5165\uff01<\/span><\/p>\n


                <\/span><\/p>\n

                <\/section>\n

                <\/span><\/p>\n


                <\/span><\/p>\n

                \u70b9\u51fb\u201c\u9605\u8bfb\u539f\u6587\u201d\uff0c\u62a5\u540d\u8bfb\u4e66\u4f1a<\/span><\/span><\/section>\n

                <\/mp-style-type><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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